[RASMB] parameters of beta-mercaptoethanol?

[Jo Butler]

Dear Joy,

Not directly answering your question, but just to point out that people 
usually avoid beta-mercaptoethanol because of its UV absorption.  While 
it may be fine with interference optics, it was no use with the older 
absorbance, nor can one use D280 to get the initial protein 
concentration.  this is why DTT is preferred - and this is in the 
Sednterp parameters.

Jo

joylo at gate.sinica.edu.tw wrote:
> Dear all
>   I have the Sednterp (version 1.09) and it does not have any information for beta-mercaptoethanol. I used two buffer systems. One included 50mM Tris and 2mM b-ME at pH 7.5, and another was 50mM Tris and 10mM b-ME at pH 7.5. Could anyone help me get the parameters needed to include the b-ME used in my buffers?
> Thanks
> Joy Lo  
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-- 
Dr P.J.G. Butler,
MRC Laboratory of Molecular Biology,
Hills Road, Cambridge, CB2 2QH, UK.
Tel. +44 (0)1223 402296