[RASMB] DTT for equilibrium runs?

[Peter Schuck]

Debby,
in my experience, a few mM DTT has not really been a big problem in the 
past.  Yes, the baseline absorbance changes with time, and it's important 
to account for that in the data analysis.  With long-column sedimentation 
equilibrium (4-5mm columns) and appropriate rotor speed that include close 
to meniscus depletion, that's not an issue, though.  TCEP is recommended by 
many colleagues, but some colleagues here have made poor experience with it 
for particular proteins.  I have not tried it, yet.
Peter

At 01:59 PM 6/6/2006, you wrote:
>Hi, everyone,
>
>I just heard recently that it's not good to have DTT in your buffer for 
>doing equilibrium runs. Can someone explain to me why that would be? Or 
>share stories of DTT use?
>
>Thanks very much,
>
>Debby
>
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