[RASMB] Use of SDS to study peptide

[Karen Fleming]

I only did equilibrium experiments on those samples.
-K.
On Jan 12, 2006, at 12:30 PM, John E Harlan wrote:

>
> Karen-
>
> Did your non-ideality occur only in sedimentation equilibrium 
> experiments, or did you see problems in sed. velocity as well.  
>
> John
>
>
> Karen Fleming <Karen.Fleming at jhu.edu>
> Sent by: rasmb-bounces at server1.bbri.org
>
> 01/12/2006 11:29 AM
>        
>         To:        Damien Thévenin <damien at UDel.Edu>
>         cc:        rasmb at server1.bbri.org, (bcc: John E 
> Harlan/LAKE/PPRD/ABBOTT)
>         Subject:        Re: [RASMB] Use of SDS to study peptide
>
>
>
> I tried it a couple of times with glycophorin and the high charge
>  density of the micelles caused a lot of nonideality in the
>  sedimentation profiles. We could never fit the data and so abandoned
>  SDS for other detergents. The charge density also causes nonideality 
> in
>  fluorescence experiments.
>  -Karen Fleming
>  On Jan 12, 2006, at 11:04 AM, Damien Thévenin wrote:
>
>  > Hello all,
>  >
>  > I study transmembrane (TM) peptides interaction in various 
> detergents
>  > (DPC, C8E5, C14-betaine,...) and I would like to know whether SDS is
>  > an suitable detergent for AUC. Indeed, I haven't seen any paper
>  > reporting the use of SDS to study TM peptides by AUC. Furthermore, 
> SDS
>  > is my detergent of choice when I study these same peptides by CD or
>  > fluorescence. So, does SDS have properties that make it unsuitable 
> for
>  > AUC?
>  > Thanks for your help and insights.
>  >
>  > Damien
>  >
>  > ----------
>  > Damien Thévenin
>  >
>  > Department of Chemistry & Biochemistry
>  > University of Delaware
>  > Delaware Biotechnology Institute
>  > 15 Innovation way (room 146)
>  > Newark, DE 19711
>  >
>  > Phone     302.831.4410
>  > FAX         302.831.3447
>  > damien at udel.edu
>  >
>  >
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>  >
>  ****************************************************
>  Karen G. Fleming, Ph.D.
>  Assistant Professor
>  Thomas C. Jenkins Department of Biophysics
>  Johns Hopkins University
>  3400 North Charles Street
>  Baltimore, MD 21218
>
>  Voice: 410-516-7256
>  Fax:                 410-516-4118
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****************************************************
Karen G. Fleming, Ph.D.
Assistant Professor
Thomas C. Jenkins Department of Biophysics
Johns Hopkins University
3400 North Charles Street
Baltimore, MD 21218

Voice: 410-516-7256
Fax:	410-516-4118
****************************************************