[RASMB] density of L-arginine solution

[Fumio Arisaka]

Dear John,

Thank you.  I also thank Jack who suggested me to measure it,
but for the moment, I take the easier way.

John, I was able to get a copy of that article you listed.
It indeed gives the vbar at molar concentrations between 0.2 M and 1.5 M.
At 1.0 M, where we measured SV, vbar for arginine HCl is 0.690.
I calculate the density of the buffer by the equation:

      d = d0 + (1-vbarrho)C

I am not sure about the accuracy with 1 M solution, but I guess
this is the only way to do unless we measure it.
John, if you have any comments on this, I would appreciate it.

Actually, I need the viscosity of the arginine solution as well.
Right now, I am using the viscosity of Guanidine HCl for it, but that
of arginine may be quite different from this, I am afraid.

Could anybody tell me where I can find the data (viscosity of Arg)?

Thanks in advance.  Fumio


At 21:05 06/02/09 -0800, you wrote:
>Fumio, I believe you will find a value for the partial specific volume of
>arginine (the amino acid, not the residue vbar in peptides) in the following
>paper, from which you can calculate the density of arginine solutions (to
>first order in concentration).
>
>Kita, Y., Arakawa, T., Lin, T. Y., and Timasheff, S. N. (1994). Contribution
>of the surface free energy perturbation to protein-solvent interactions.
>Biochemistry 33, 15178-15189.
>
>John
>
>-----Original Message-----
>From: rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org] On
>Behalf Of Fumio Arisaka
>Sent: Wednesday, February 08, 2006 6:26 PM
>To: RASMB
>Subject: [RASMB] density of L-arginine solution
>
>
>
>Dear RASMBers,
>
>Could anybody tell me where I could find the density data
>for L-arginine solution?
>
>Thank you in advance.  Fumio
>
>
>***********************************************************
>Fumio Arisaka, Ph.D.
>Department of Biomolecular Engineering
>Graduate School of Bioscience and Biotechnology
>Tokyo Institute of Technology
>5249 Nagatsuta, Midori-ku
>Yokohama 226-8501-B39  JAPAN
>Tel/Fax:+81-45-924-5713
>E-mail: farisaka at bio.titech.ac.jp
>URL  http://www.farisaka.bio.titech.ac.jp/　　
>***********************************************************