[RASMB] density of L-arginine solution

[Steve Shire]

Dear Fumio,
      One other suggestion. Anton-Parr makes a small hand held 
viscometer based on the oscillating quartz tuning fork. This is 
actually a simplified version of the densitometer that has been used to 
determine vbar values. Although it will only give results to 3 
significant figures that is sufficient for use in sedimentation work. 
I'm not sure how much this instrument is currently but I would guess it 
is less than $500. The best thing is you can get reliable 
determinations in less than 5 minutes of work, We routinely use it for 
all our sedimentation work. Hope this is of some use.
         Steve
On Feb 10, 2006, at 8:34 PM, Fumio Arisaka wrote:

>
> Dear John,
>
> Thank you.  I also thank Jack who suggested me to measure it,
> but for the moment, I take the easier way.
>
> John, I was able to get a copy of that article you listed.
> It indeed gives the vbar at molar concentrations between 0.2 M and 1.5 
> M.
> At 1.0 M, where we measured SV, vbar for arginine HCl is 0.690.
> I calculate the density of the buffer by the equation:
>
>      d = d0 + (1-vbarrho)C
>
> I am not sure about the accuracy with 1 M solution, but I guess
> this is the only way to do unless we measure it.
> John, if you have any comments on this, I would appreciate it.
>
> Actually, I need the viscosity of the arginine solution as well.
> Right now, I am using the viscosity of Guanidine HCl for it, but that
> of arginine may be quite different from this, I am afraid.
>
> Could anybody tell me where I can find the data (viscosity of Arg)?
>
> Thanks in advance.  Fumio
>
>
> At 21:05 06/02/09 -0800, you wrote:
>> Fumio, I believe you will find a value for the partial specific 
>> volume of
>> arginine (the amino acid, not the residue vbar in peptides) in the 
>> following
>> paper, from which you can calculate the density of arginine solutions 
>> (to
>> first order in concentration).
>>
>> Kita, Y., Arakawa, T., Lin, T. Y., and Timasheff, S. N. (1994). 
>> Contribution
>> of the surface free energy perturbation to protein-solvent 
>> interactions.
>> Biochemistry 33, 15178-15189.
>>
>> John
>>
>> -----Original Message-----
>> From: rasmb-bounces at rasmb.bbri.org 
>> [mailto:rasmb-bounces at rasmb.bbri.org] On
>> Behalf Of Fumio Arisaka
>> Sent: Wednesday, February 08, 2006 6:26 PM
>> To: RASMB
>> Subject: [RASMB] density of L-arginine solution
>>
>>
>>
>> Dear RASMBers,
>>
>> Could anybody tell me where I could find the density data
>> for L-arginine solution?
>>
>> Thank you in advance.  Fumio
>>
>>
>> ***********************************************************
>> Fumio Arisaka, Ph.D.
>> Department of Biomolecular Engineering
>> Graduate School of Bioscience and Biotechnology
>> Tokyo Institute of Technology
>> 5249 Nagatsuta, Midori-ku
>> Yokohama 226-8501-B39  JAPAN
>> Tel/Fax:+81-45-924-5713
>> E-mail: farisaka at bio.titech.ac.jp
>> URL  http://www.farisaka.bio.titech.ac.jp/　　
>> ***********************************************************
>
>
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>
Steven J. Shire, Ph.D.
Staff Scientist and Group Leader
Late Stage Pharmaceutical and Device Development
MS #96A
Genentech, Inc.
1 DNA Way
S. San Francisco, CA 94080
650-225-2077 (VOICE)
650-225-7234 (FAX)
shire.steve at gene.com (E-mail)

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