[RASMB] peak in sedimentation velocity scans at high protein conc.

[Andy Baron]

Norbert,
In the early scans of the highest protein samples it looks as if there 
is a very steep boundary which deflects the incident light, causing 
the spike. I would be surprised if 6g/l protein produced such a 
steep boundary; what was in your buffer? (We saw such a 
phenomenon when spinning a sample in the presence of 0.1% 
detergent. The micelles produced a displacement of 70 fringes, and 
spikes in the absorbance scans. See attached gif file.)

Cheers,
Andy



Andy Baron
Astbury Centre for Structural Molecular Biology
Faculty of Biological Sciences
University of Leeds
LEEDS
LS2 9JT
UK

Tel:    +44 (0)113 34 37278
Fax:    +44 (0)113 34 31407
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