[RASMB] Am I missing something?
Walter Stafford
stafford at bbri.org
Fri Jun 10 23:03:00 PDT 2005
Hi Dror,
You can double your through-put by putting samples into both
sectors of a single cell and collecting intensity data. There are at
least 2 programs that can process intensity data into
pseudo-absorbance data: SEDFIT by Peter Schuck and SEDANAL by
Sherwood & Stafford can both handle intensity data in various types
of analysis.
Walter
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>Dear all,
>I'm running sedimentation velocity experiments on our XLA where a
>typical scan (no replicates, r=6.0 to 7.2) takes about 1 min. If I
>try to scan two cells or more, the time interval between scans
>increases proportionally because the XLA software scans the cells
>one at a time (i.e. scans the whole radial range for cell 1 then
>cell 2 etc.). This makes scanning several cells unreasonably slow.
>Does anybody know if it's possible to scan multiple cells
>simultaneously a radial step at a time (i.e. measure at r=x for all
>the cells then for r = x+dx for all cells etc.)?
>Thanks,
>Dror
>=========================
>Dror Noy, Ph.D.
>Structural Biology Dept.
>Weizmann Institute of Science
>Rehovot 76100
>Israel
>
>e-mail: dror.noy at weizmann.ac.il
>Tel: (972)-8-934 2525
>Fax: (972)-8-934 4154
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Walter Stafford
mailto:stafford at bbri.org
direct dial: 617-658-7808
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