[RASMB] Origin software and vbar

[John Philo]

Sorry Jack, but I have to disagree with you. First, you are talking about
velocity analysis, not equilibrium. The Beckman software is by far the most
user-friendly equilibrium analysis package I have seen. Its algorithms are
equivalent to those in NONLIN, but you don't have to fight with obscure
speed factors etc., and it actually gives you answers in real molecular
weight (at least when it works correctly), which NONLIN never does. Yes,
there are more sophisticated things that can be done, but if you don't need
them, why struggle? 
 
Second, what is wrong with simple? Isn't it particularly important for new
users to achieve some quick success so they are encouraged to do more,
including learning these 'powerful and elegant' approaches?
 
John

-----Original Message-----
From: rasmb-admin at server1.bbri.org [mailto:rasmb-admin at server1.bbri.org] On
Behalf Of John Correia
Sent: Wednesday, May 04, 2005 11:34 AM
To: rasmb at server1.bbri.org; apl3 at york.ac.uk
Cc: John.McGeehan at port.ac.uk
Subject: Re: [RASMB] Origin software and vbar


This may seem to be an odd question, but why are you using the Beckman
Origin software?  Depending upon your guru (Schuck & sedfit or Stafford &
SEDANAL or Demeler & Ultrascan or ... ) there are incredible packages being
developed out there - & they all work when applied to appropriate systems -
we use a combination of c(s) g(s) to establish a hypothesis, maybe some
weight average fitting to explore energetics, and then global direct
boundary fitting to hetero models with SEDANAL as the final step!  I realize
Beckman is trying to make it uniform & simple, but with the versatility,
power and elegance of these new analysis methods, why would you settle for
simple?
 
-------------------------------------------------------------------
 Dr. John J. "Jack" Correia
 Department of Biochemistry
 University of Mississippi Medical Center
 2500 North State Street
 Jackson, MS  39216
 (601) 984-1522                                 
 fax (601) 984-1501                             
 email address: jcorreia at biochem.umsmed.edu     
 homepage location: http://biochemistry.umc.edu/correia.html
 dept homepage location:    http://biochemistry.umc.edu/
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