[RASMB] membrane protein LS contribution

[Ewa Folta-Stogniew]

--- Ariel Lustig <ariel.lustig at bluewin.ch> wrote:

> Dear colleauges,
> Mainly for  those who are  interested in membrane
> proteins.
>  For the  determination of  of  the  molecular 
> masses of  membrane  proteins (MP) are used
> different methodes,
> some are complicated  to  run  or /and to calculate 
> the the  protein-only  of the detergent complex. 
(...)

> L-Scattering give only  averages.


just a comment on LS contribution to analysis of
membrane proteins; it can do much better than detect
aggregates and it can do that in tima comparable to
regular SEC analysis.

HAYASHI,Y., H.MATSUI & T.TAKAGI. 1989. Membrane
protein molecular weight determined by low-angle laser
light-scattering photometry coupled with
high-performance gel chromatography Methods Enzymol.
172:514-28.: 514-528.


YERNOOL,D., O.BOUDKER, E.FOLTA-STOGNIEW & E.GOUAUX.
2003. Trimeric subunit stoichiometry of the glutamate
transporters from Bacillus caldotenax and Bacillus
stearothermophilus Biochemistry 42: 12981-12988.

WEI,Y., H.LI & D.FU. 2004. Oligomeric State of the
Escherichia coli Metal Transporter YiiP J. Biol. Chem.
279: 39251-39259.

Ewa













> The  sucrose for matching  the detergent  micelles
> causes to strong  hydration of the detergent-micell.
> Incase  of 
> DM from ro 1.22 to 1.12.
> and the  protein (as longtime  was known with high
> molar salt  that the shift  may be  from ro 1.35 to
> 1.28  about) so the  hydration as we corrected it is
> not so precise but helps for  the  contribution of
> the shown papers.
> yours ,,,,   ariel
>  > _______________________________________________
> RASMB mailing list
> RASMB at rasmb.bbri.org
> http://rasmb.bbri.org/mailman/listinfo/rasmb
> 



		
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