[RASMB] Origin software and vbar

[John Correia]

That is why I asked John.  SEDANAL & I believe Jim's progam fit both
velocity and equilibrium data to MW too!  The major issue is
hetero-analysis - does the Beckman software do hetero?


>>> "John Philo" <jphilo at mailway.com> 05/04/05 04:19PM >>>

Sorry Jack, but I have to disagree with you. First, you are talking
about velocity analysis, not equilibrium. The Beckman software is by far
the most user-friendly equilibrium analysis package I have seen. Its
algorithms are equivalent to those in NONLIN, but you don't have to
fight with obscure speed factors etc., and it actually gives you answers
in real molecular weight (at least when it works correctly), which
NONLIN never does. Yes, there are more sophisticated things that can be
done, but if you don't need them, why struggle? 
 
Second, what is wrong with simple? Isn't it particularly important for
new users to achieve some quick success so they are encouraged to do
more, including learning these 'powerful and elegant' approaches?
 
John


-----Original Message-----
From: rasmb-admin at server1.bbri.org
[mailto:rasmb-admin at server1.bbri.org] On Behalf Of John Correia
Sent: Wednesday, May 04, 2005 11:34 AM
To: rasmb at server1.bbri.org; apl3 at york.ac.uk 
Cc: John.McGeehan at port.ac.uk 
Subject: Re: [RASMB] Origin software and vbar


This may seem to be an odd question, but why are you using the Beckman
Origin software?  Depending upon your guru (Schuck & sedfit or Stafford
& SEDANAL or Demeler & Ultrascan or ... ) there are incredible packages
being developed out there - & they all work when applied to appropriate
systems - we use a combination of c(s) g(s) to establish a hypothesis,
maybe some weight average fitting to explore energetics, and then global
direct boundary fitting to hetero models with SEDANAL as the final step!
 I realize Beckman is trying to make it uniform & simple, but with the
versatility, power and elegance of these new analysis methods, why would
you settle for simple?
 
-------------------------------------------------------------------
 Dr. John J. "Jack" Correia
 Department of Biochemistry
 University of Mississippi Medical Center
 2500 North State Street
 Jackson, MS  39216
 (601) 984-1522                                 
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 email address: jcorreia at biochem.umsmed.edu     
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 dept homepage location:    http://biochemistry.umc.edu/ 
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