[RASMB] Stiff equilibrium centrepieces

[straussh at gmx.de]

Andrew,

I wonder wether the prolonged use of ethanol wouldn't cause the flexibiliser
of the CPs to be washed out, and/or the ethanol to diffuse into the epon and
thus cause the swelling-cum-stiffness? That problem would be more pronounced
with 6-channel CPs because of their large surface/volume ratio. 
Maybe placing the CPs in an exsiccator over Na or something else absorbing
ethanol could help? Alternatively, grinding some material off the outside of
the CP?

Cheers,
Holger


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> 
> Hello all from the gloomy north of England,
> 
> I'm having some trouble with my equilibrium centrepieces - they are
> getting more and more tight in the housings. I don't think we are
> doing anything unusual with them - they are washed carefully in dilute
> decon, water and finally rinsed with ethanol and left to dry. There
> is no obvious deposit or change to the plastic (charcoal filled epon).
> Velocity centrepieces seem to fit ok in the housings so I don't think
> they are the problem.
> 
> Has anybody had the same difficulty, and even better, does anybody have
> a remedy for obstinate centrepieces?
> 
> Many thanks,
> 
> Andrew
> 
> -- 
> Dr Andrew Leech                   *  Laboratory Manager
> Technology Facility               *  Molecular Interactions Laboratory
> Department of Biology (Area 15)   *  Tel   : +44 (0)1904 328723
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