[RASMB] FW: interference optics

Fasolini, Marina [ITCRE/1011] marina.fasolini at nervianoms.com
Fri Jun 18 05:46:01 PDT 2004


> Dear AUC experts
> 
> We are new to AUC. We were trying to do a velocity run with both
> interference and absorption optics. The analysis of the results looked
> good when we looked at the absorption scans, however we have not managed
> to get good results with the interference scans. Using the dcdt program we
> observe that the plot of dcdt vs S instead of being a normal gaussian is
> extremely bumpy. We have also not managed to get a result using SEDFIT
> whereas, as I said before the data looks very nice with the absorption
> scans. Does anybody have any comments. Is it likely to be the analysis of
> the results, something wrong with the optics or something we are doing
> wrong in the experimental setup? (To verify we weren't doing something
> drastically wrong in the programs we took Peter Schuck's data from his web
> site and analysed it with both programs and we didn't have any problems).
> We also notice that the position of the meniscus is slightly different in
> the two optics systems (6.288 vs 6.228) but maybe this is to be expected. 
> 
> Thanks in advance for your help.
> 
> Marina Fasolini
> NERVIANO MEDICAL SCIENCE
> Structural Chemistry 
> Viale Pasteur 10
> 20014 Nerviano (Mi) - Italy
> Tel. +39 0331 581462
> Fax +39 0331 581317
> marina.fasolini at nervianoms.com
> 



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