[RASMB] 4 mm center-piece

[Emmanuel Guigard]

Hi all,

In order to analyze protein samples at higher (NMR) concentrations, the
previous XL-I operator used a modified a center-piece that was reducing the
path length from 1.2 to 0.4 cm.

After running two sets of sedimentation equilibrium experiments, the Mw
average obtained from the 0.4 path length center-piece seems to always be
about 3 to 4 times less than the Mw average obtained from a regular
center-piece.

Does anyone performed this kind of runs ?  If so, do one needs to "adjust"
the data before analyzing then with Sednterp ?   Multiplying the number of
fringes by 4 before calculating the Mw average did not change the results.
Also, no cell leakages from the modified center-piece were observed.

Thank you in advance for your help.

Emmanuel


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Emmanuel Guigard, B.Sc.
Research Assistant
Protein Engineering Network of
Centres of Excellence;

Department of Biochemistry
Rm 3-34 Medical Sciences Bldg.
University of Alberta
Edmonton, AB.
T6G 2H7

Ph : (780)-492-3412
FAX : (780)-492-0095
email : eguigard at biochem.ualberta.ca
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