[RASMB] glycosylation in sigma value

Ghirlando, Rodolfo (NIH/NIDDK) RodolfoG at intra.niddk.nih.gov
Fri Apr 2 11:35:00 PST 2004


In addition please note the following references, in which all components
are glycosylated. In particular note the ~25% and 40% carbohydrate mass
content of FcgammaRIII and FcepsilonRIalpha, respectively.	

Ghirlando, R., Keown, M. B., MacKay, G., Lewis, M. S., Unkeless, J. C. &
Gould, H. J. (1995). Stoichiometry and thermodynamics of the interaction
between the Fc fragment of human IgG1 and its low-affinity receptor
FcgammaRIII. Biochemistry 34, 13320 - 13327. (Equations 6 and 7).

Keown, M. B., Ghirlando, R., Young, R. J., Beavil, A. J., Owens, R. J.,
Perkins, S. J., Sutton, B. J. & Gould, H. J. (1995). Hydrodynamic studies of
a complex between the Fc-fragment of human IgE and a soluble fragment of the
FcepsilonRIalpha-chain. Proceedings of the National Academy of Sciences
U.S.A. 92, 1841 - 1845.

Kind regards, Rodolfo.

Rodolfo Ghirlando
LMB-NIDDK-NIH
Department of Health and Human Services
Bldg. 5, Room 208
5 Center Drive MSC 0540
Bethesda, MD 20892-0540
USA

Tel: 301-496-5889
Fax: 301-496-0201
e-mail: rodolfo.ghirlando at nih.gov


        





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