[RASMB] velocity or equilibrium?

[Patrick Brown]

Dear all:

I would like to study a heterodimerization process
between protein A(35 kDa) and protein B (9.5 kDa). 
Protein A also dimerizes (Kd = 35 microM).

Which method (sed. equilibrium or velocity)would
provide the most accurate estimate of the Kd?

And, is there a software package (ie. sedfit, dc/dt,
Nonlin, sedanal, etc.) that will allow for
deconvolution of the hetero vs. homo dimerization?

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