[RASMB] 2 ligands and no equation

[Arthur Rowe]

Holger -

Happy New Year to you and all RASMBers !

As regards your problem, it seems to me that doing maths on dynamic
processes is boring - and hardly necessary when there is plenty of software
which "is designed to facilitate the mapping, modeling and simulating of
dynamic processes". To quote from the web site of those who make and market
my own favoured product (STELLA, from High Performance Systems, Inc:
http://www.hps-inc.com/index.htm, click on STELLA in the LH menu).

Of course there are other programs out there, but STELLA is long
established, is both Mac and PC compatible (and models can be transferred
across the platforms), and does not take lots of learning to get output.
Although a lot of its applications are social science based, a rate is a
rate, and it's easy to set a model* up for a multi-component interacting
system.

Just a thought

Arthur

e.g. Alistair McGregor, Andrew D Blanchard, Arthur J Rowe and David R
Critchley (1994) Identification of the vinculin binding site in the
cytoskeletal protein a-actinin  Biochem  J  301  225-233

--
*******************************************************
Arthur J Rowe
Professor of Biomolecular Technology
NCMH Business Centre
University of Nottingham
School of Biosciences
Sutton Bonington
Leicestershire LE12 5RD   UK

Tel:        +44 (0)115 951 6156
            +44 (0)116 271 4502
Fax:        +44 (0)115 951 6157
email:      arthur.rowe at nottingham.ac.uk
            arthur.rowe at connectfree.co.uk (home)
Web:        www.nottingham.ac.uk/ncmh/business
*******************************************************


From: Holger Strauss <strauss at fmp-berlin.de>
Date: Mon, 13 Jan 2003 11:47:43 +0100 (MET)
To: mail-to-all at rasmb <rasmb at rasmb-email.bbri.org>
Subject: [RASMB] 2 ligands and no equation


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Hello everbody,

the new year has hardly begun, the institute slowly gets back to live, and
we are stuck with the following problem: we have a protein and two
ligands, both binding to the same binding site on the protein. We know
both the individual Kds (mikromolar range) of the ligands binding to the
protein. Now, the question is, how can
one describe the concentrations of all species (bound/free ligand A,
bound/free ligand B, free protein), when both ligands are
present at about equal concentrations (+- one order of magnitude) together
with the protein? Our efforts so far landed us with some horrifying
equations with an equally horrifying number of solutions... but there must
be a solution to the problem, must there not? All comments are greatly
appreciated!

Greeting and happy New Year to all, Holger

- - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Holger Strauss 

Forschungsinstitut fuer Molekulare Pharmakologie (FMP)
Robert-Roessle Strasse 10

13125 Berlin/Germany

Tel: +49 (0)30 94793 - 223 (office)
                    - 316 (lab)

Fax: +49 (0)30 94793 - 169


- - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Science is spectrum analysis; art is photosynthesis.

                                                   Karl Kraus

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