[RASMB] 2 ligands and no equation

Holger Strauss strauss at fmp-berlin.de
Mon Jan 13 05:48:01 PST 2003


Hello everbody,

the new year has hardly begun, the institute slowly gets back to live, and
we are stuck with the following problem: we have a protein and two
ligands, both binding to the same binding site on the protein. We know
both the individual Kds (mikromolar range) of the ligands binding to the
protein. Now, the question is, how can
one describe the concentrations of all species (bound/free ligand A,
bound/free ligand B, free protein), when both ligands are
present at about equal concentrations (+- one order of magnitude) together
with the protein? Our efforts so far landed us with some horrifying
equations with an equally horrifying number of solutions... but there must
be a solution to the problem, must there not? All comments are greatly
appreciated!

Greeting and happy New Year to all, Holger

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Holger Strauss 

Forschungsinstitut fuer Molekulare Pharmakologie (FMP)
Robert-Roessle Strasse 10

13125 Berlin/Germany

Tel: +49 (0)30 94793 - 223 (office)
                     - 316 (lab)

Fax: +49 (0)30 94793 - 169 


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Science is spectrum analysis; art is photosynthesis.

                                                    Karl Kraus




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