[RASMB] sedimentation

[Arthur Rowe]

Hi Irene

There is a long history of using the AUC to look at PSL particles, starting
(I think) with a lovely paper by Cheng & Schachman (1955) J Polym Sci 16:19+
which established what is still the most precise reference data for
validating the application of Stokes Law to small spheres, as well as
characterising the s-c dependence of such spheres.

As the density of PSL is quite close to 1, you can get away with looking
much larger PSL particles in the AUC than you could with denser (e.g.
protein-based) material. As you have the freedom to adjust the density
difference and make it even smaller by adding (say) salt or deuterium oxide
to the solvent, I doubt that there is any real upper limit to the size of
spheres you can cope with, when running the AUC at low speeds.

All best wishes for your work

Regards

Arthur


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Arthur J Rowe
Professor of Biomolecular Technology
NCMH Business Centre
University of Nottingham
School of Biosciences
Sutton Bonington
Leicestershire LE12 5RD   UK

Tel:        +44 (0)115 951 6156
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email:      arthur.rowe at nottingham.ac.uk
            arthur.rowe at connectfree.co.uk (home)
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From: "Irene Hallyburton" <i.hallyburton at dundee.ac.uk>
Organization: Dundee University
Date: Fri, 24 Oct 2003 12:09:48 GMT
To: rasmb at rasmb-email.bbri.org
Subject: Re: [RASMB] sedimentation


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Hi All

In the same vein as Jan's silica particles, a client has been using latex
microparticles in the development of Point of Care assays. He has
asked me about looking at them, coated and uncoated, in the AUC.
They will be quite large, and we did consider DLS, but our instruments
max is 50nm and they'll be bigger than that.

What is the upper size range for AUC?

Many Thanks

Irene



<0>-<0>-<0>-<0>-<0>-<0>-<0>-<0>-<0>-<0>-<0>
Irene Hallyburton
Post Genomics & Molecular Interactions Centre
Faculty of Life Sciences
University of Dundee
01382 348700


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