[RASMB] RE: Scans

[Jones, Samantha]

I also should have mentioned that I was spinning at 50,000rpm, over a couple
of hours
The magnitude of the change of the scans is slight when compared to the
concentration of protein. 


Samantha

PS Thanks to John Philo!
> -----Original Message-----
> From:	Jones, Samantha 
> Sent:	22 July 2003 12:16
> To:	'rasmb at rasmb-email.bbri.org'
> Subject:	Scans
> 
> Hello
> I wonder if any one can give some advice.
> I have been doing some SV runs on my 16Kd protein that has been dialysed
> overnight in buffer with 150mM DTT. I have been overlaying the scans in
> Sedfit and when I see the scans overlaid I cannot understand exactly what
> is happening. 
> Instead of a moving boundary the scans are all a curve from the point of
> meniscus to the bottom of the cell which becomes slightly more steeper
> over time, rotating around a point three quarters of the way along the
> radius of the cell. There is no clearing of the meniscus, no plateau and
> the scans are bunched around four different fringe displacement values.
> This pattern of scans has been seen with various concentration of the
> protein and even with different DTT concentrations.
> I am not sure if it is possible to attach the display of the scans to an
> email from Sedfit, as this would have made it a bit easier to describe. 
> 
> Thank you
> 
> 
> Samantha 
> 
> 
> Samantha Jones
> MRC Prion Unit
> Imperial College School of Medicine at St Marys
>