[RASMB] His tagged proteins

[John Rodgers]

I can imagine problems with performing ultracentrifugation experiments with
His tagged proteins.  It seems that this was also mentioned in the recent
workshop. A set of equilibrium runs were made before the workshop with the
more abundant and available His tagged protein provided by the researcher.
With regard to the protein,  SEC static light scattering experiments were
performed on both native extracted proteins and His tagged protein with
consistent results (dimer-?tetramer in rapid equilibrim.).    The researcher
makes the argument that the His tags are of no significant consequence, but
is open to doing the extra work if a good case can be made for doing so.
Experience, advice and references appreciated.  
Thanks,
John Rodgers  
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