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<DIV><SPAN class=112312921-08072003><FONT face=Arial size=2>I can imagine
problems with performing ultracentrifugation experiments with His tagged
proteins. It seems that this was also mentioned in the recent
workshop. A set of equilibrium runs were made before the workshop with
the more abundant and available His tagged protein provided by the
researcher. With regard to the protein, SEC static light
scattering experiments were performed on both native extracted proteins and His
tagged protein with consistent results (dimer-?tetramer in rapid
equilibrim.). The researcher makes the argument
that the His tags are of no significant consequence, but is open
to doing the extra work if a good case can be made for doing so.
Experience, advice and references appreciated. </FONT></SPAN></DIV>
<DIV><SPAN class=112312921-08072003><FONT face=Arial
size=2>Thanks,</FONT></SPAN></DIV>
<DIV><SPAN class=112312921-08072003><FONT face=Arial
size=2>John Rodgers </FONT></SPAN></DIV></FONT></DIV></BODY></HTML>