[RASMB] Frict ratios, multiple species, and sedfit

[Avi Chakrabartty]

Dear RASMB,

We are using sedfit to examine a self-associating system. We find that the
monomer molecular weight is reasonably accurate, however, the dimer weight
is lower than expected and the tetramer weight is even lower still. Could
this be the result of differences in the axial ratios of the oligomers,
with the higher oligomers being more rod-like than the smaller oligomers.
If this is the case, can the frictional ratios for different sedimenting
species be floated in sedfit.

Thanks, Avi

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Avi Chakrabartty, Ph.D., Associate Professor
Departments of Medical Biophysics and Biochemistry,
University of Toronto, Ontario Cancer Institute,
610 University Avenue, Toronto, Ontario, M5G 2M9, CANADA

Phone:416-946-4501 (office:ext. 4910) (lab:ext. 4915), FAX: 416-946-6529
email: chakrab at uhnres.utoronto.ca
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