[RASMB] liposomes

[Lustig]

Dear Patric  and  colleagues,
from my  experience, usually are liposomes not homogene in size  and  density.
First one have to ask about the  density , because  without  density no 
ultracentrifugation has a
meaning!!!!

The best methode    is the  determination  using   S' eta = viscosity 
versus density, in this case
D2O +buffer is the best medium  I know. Densities will be  close to 10-50%D2O

  An exelent paper from C H Huang ang J P Charlton 1971= "Studies of 
Phosphat. vesicles" JBC
vol246 N°8 2555-2560.
The system in your case has to be  adapted  to ethanol/.H2O......yours  ariel

Lustig Ariel
Biozentrum der Universität Basel
Klingelbergstr. 70
CH 4056 Basel
Switzerland
Dept. of Biophysical Chemistry  /
Analytical Ultracentrifuge Lab.TEL....+61 2672187
                                Fax    +61 2672189
E-mail   Ariel.Lustig at unibas.ch