[RASMB] detergent for AUC

[Joachim Behlke]

An:             	rasmb at rasmb-email.bbri.org
Von:            	Karsten Rippe <karsten.rippe at dkfz-heidelberg.de>
Betreff:        	[RASMB] detergent for AUC
Datum:   	Mon, 11 Nov 2002 15:48:39 +0100 (CET)

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 Dear Karsten and colleages,

Lubrol PX or dodecyl-PEG-(9-10)ether is a detergent whithout 
absorbance in the range 280-230 nm. We have used it for the 
disaggregation of proteins (see the Harding et al. book 1992).
The CMC is about 40 microM.

Best wishes

Joachim Behlke

> I am looking for a detergent that can be used in analytical ultracentrifugation
> runs of a protein with absorbance detection at 280 and 230 nm. The protein is
> currently purified in the presence of 0.1% NP 40 which has a strong UV
> absorbance so that UV detection is not possible. Can anyone recommend a
> replacement for NP-40? Thanks a lot.
> 
> Best regards,
> 
> Karsten Rippe
> 
> 
> -----------------------------------------------------------------
> PD Dr. Karsten Rippe
> Kirchhoff-Institut für Physik
> Molekulare Biophysik (F15)
> Universität Heidelberg
> Im Neuenheimer Feld 227
> and
> Deutsches Krebsforschungszentrum
> Molekulare Genetik (H0700)
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> D-69120 Heidelberg
> Germany
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