[RASMB] hydrostatic pressures

[John Correia]

It might be worthwhile looking at the papers Gerson Kegeles published on
pressure effects - since the effect depends upon the Molar volume of
reaction small pressures can have large effects & I suspect 1000's of
bar may not be required, ie. do you have to redesign the cell?   Plus
Keg in collaboration with Raymond Kikas from Dave Yphantis' lab built a
chamber for pressurizing cells that I think Tom Laue inherited?

Saxena et al 1976 Biophys Chem 5: 161-164

Kegeles & Johnson (1970) ABB 141: 59-62

Kegeles & Johnson (1970) ABB 141: 63-67

Kegeles (1970) ABB 141: 68-72

Kegels et al 1969  ANYAS 164: 183-191

There are also many papers by Harrington on pressure effects in myosin
& the construction of a different bomb for filling cells.  There bomb
swelled and thus really was a bomb waiting to blow.

Finally, Walter Stafford has published simulations of pressure effects
for interacting systems.

-------------------------------------------------------------------
 Dr. John J. "Jack" Correia
 Department of Biochemistry
 University of Mississippi Medical Center
 2500 North State Street
 Jackson, MS  39216
 (601) 984-1522                                 
 fax (601) 984-1501                             
 email address: jcorreia at biochem.umsmed.edu     
 homepage location: http://biochemistry.umc.edu/correia.html  
 dept homepage location:    http://biochemistry.umc.edu
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>>> JA KORNBLATT <krnbltt at vax2.concordia.ca> 05/07/02 01:19PM >>>
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the two kornblatts have worked with hydrostatic pressure as a
perturbant
for a long time and with the auc for only a couple of years.

many of the changes induced by hydrostatic  pressures in the range of
1
bar to  4000 bar would be "easily" detected by auc analysis. this
includes
shape changes such as the open/closed transition of plasminogen,
association/dissociation reactions, and native/denatured reactions.

the question: has anyone spent any idle moments reflecting on the
question
of how much effort might be expended on building an auc cell that
would
withstand a couple of thousand atmospheres pressure?
am i wasting the few remaining neurons thinking about the prospects or
would it be worth while giving it some effort?
any help you can give would be appreciated.
best regards
jack (kornblatt)

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