[RASMB] very slow sedimenting protein

Virgil Schirf schirfv at bioc09.v19.uthscsa.edu
Thu Aug 1 06:01:00 PDT 2002


Good morning!

We have a protein with a Mr = 15570 (and was expected to form a tight 
dimer) and performed a sedimentation velocity experiment. After 4 hours 
at 60000 rpm, a slight shoulder formed as the boundary, but it never 
cleared the meniscus. It was run at 20 deg C in 100 mM phosphate buffer. 
The metal containing form of the protein ran as expected. The question 
is: why does the apo form of the protein not sediment? One theory is 
that it exists as a random coil without secondary structure. Does anyone 
have any experience with a protein such as this.

Thanks to all,

Virgil
-- 
Virgil Schirf
Department of Biochemistry, Rm 535C
The University of Texas Health Science Center at San Antonio
7703 Floyd Curl Dr.
San Antonio TX 78229
email: schirfv at biochem.uthscsa.edu
Voicemail: (210) 567-6590  FAX: (210)567-6595
http://cauma.uthscsa.edu




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