[RASMB] Overspeeding to establish equilibrium

[Allen P. Minton]

Hello Andrew and Rasmbers:

The most extensive systematic study of overspeeding of which I am aware
was done by Ron Chatelier when he was a postdoc in my lab back in the late
80's.  His results were published in Analytical Biochemistry, and the
abstract of his article is attached.  He found that with careful control
of overspeeding time and velocity it is possible to shorten the time to
sedimentation equilibrium by up to about five-fold.  However, overspeeding
is a two-edged sword.  If you overspeed too much, you can actually
substantially retard attainment of equilibrium.
Caveat lector!

Allen Minton

> Is there a procedure, or rule of thumb, to decide how fast to
> run the AUC to minimise the time taken to reach equilibrium?
>
> I am thinking about unstable samples and how to avoid the long
> delays before reaching equilibrium.




-------------- next part --------------
An embedded and charset-unspecified text was scrubbed...
Name: chatelier.txt
URL: <http://list.rasmb.org/pipermail/rasmb-rasmb.org/attachments/20020626/7a10782f/attachment.txt>