[RASMB] SEC Simulations

[H. Olin Spivey]

Dear Colleagues,

    Another question placed in a separate message to facilitate indexing.

    We have extensive data testing for association between two 
different enzymes on a size exclusion HPLC column using the modified 
Hummel-Dreyer technique of Gegner and Dahlquist (1991) Proc. Natl 
Acad. Sci. U.S.A. 88, 750-754.  We perform the elutions with a series 
of ligand concentrations, which absorb at 280 and 340 nm.  Thus the 
elution profiles at 280 and 340 nm are quite complex.  We would like 
to have a simulation program to check our interpretation of these 
profiles.  Would some of you be able to recommend such a program? 
Although it would be nice to include diffusion, a program ignoring 
diffusion would still be helpful.  It would be  nice to include 
association equilibria, but a program without this ability is better 
than no program.

Thank you,
Olin
-- 
H. Olin Spivey                       Phone: (405) 744-6192
Dept. Biochem. & Molec. Biology      Fax:   (405) 744-7799
246 NRC                              Email: OSpivey at Biochem.Okstate.Edu
Oklahoma State University
Stillwater, OK 74078-3035