[RASMB] Beckman Analysis Software

Jo Butler pjgb at mrc-lmb.cam.ac.uk
Tue Oct 23 09:03:04 PDT 2001


Dear Kristian,

One obvious point from your mail is that you do not need the wavelength as 
such - it is incorporated into the refractive increment as this will be 
wavelength dependent and you will need to use the correct one.

Jo

--On Tuesday, October 23, 2001 5:17 pm +0200 Kristian Schilling 
<schilling at nanolytics.de> wrote:

> The older archived RASMB emails can be found at:
>
> http://rasmb-email.bbri.org/rasmb_archives
>
> and current archives at
>
> http://rasmb-email.bbri.org/pipermail/rasmb/
>
> -------------------------------------------------------------------------
> ---------
>
> Hello out there,
>
> in the Beckman analysis software package, there is a simulator for
> equilibrium data. To get the correct fringe displacement for a given
> concentration, you need to enter a conversion factor.
>
> Common sense says that this factor should contain the refractive index
> increment, the optical pathlength and the laser wavelength. However, my
> efforts to make a connection between the conversion factor and these
> variables have been fruitless.
>
> Does anybody know how the conversion factor is defined?
>
> Thanks for any suggestions,
>
> Kristian
>
>
>
> =================================
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P.J.G. Butler,
MRC Laboratory of Molecular Biology,
Hills Road, Cambridge, CB2 2QH, UK.
Tel. +44 (0)1223 402296



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