[RASMB] still Beckman software

Kristian Schilling schilling at nanolytics.de
Tue Oct 23 09:41:12 PDT 2001


John,

have you actually seen this formula work quantitatively? In the sense, 
perhaps, that the fringe number you get in the simulation actually matches 
fringes measured for that material.

The factor of 3.3 you give would fit for 1,2 cm pieces, 675 nm light and 
dn/dc = 0.190 ml/g, it would be in mg/ml. If I do a simulation with 
meniscus depletion, say a 5000 Da species @ 60k, I get about 250 fringes at 
the cell bottom for 10 mg/ml. There is no Ja here, so it is fairly easy to 
calculate an initial loading concentration, which would be about 150 
fringes. Such a sedimentation boundary would mean 44 mg/ml under the given 
conditions. And not 10.

Either I am doing something terribly wrong or there is some additional 
factor in there.

Kristian (not yet happy)




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