<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<meta http-equiv="Content-Type" content="text/html; charset=us-ascii">
<meta name="Generator" content="Microsoft Word 15 (filtered medium)">
<style><!--
/* Font Definitions */
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
@font-face
{font-family:Cambria;
panose-1:2 4 5 3 5 4 6 3 2 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0in;
font-size:11.0pt;
font-family:"Calibri",sans-serif;}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:blue;
text-decoration:underline;}
.MsoChpDefault
{mso-style-type:export-only;
font-size:10.0pt;}
@page WordSection1
{size:8.5in 11.0in;
margin:1.0in 1.0in 1.0in 1.0in;}
div.WordSection1
{page:WordSection1;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]-->
</head>
<body lang="EN-US" link="blue" vlink="purple" style="word-wrap:break-word">
<div class="WordSection1">
<p class="MsoNormal">Thank you both!<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I think you are exactly right, it was crud on the optics.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Jeff<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<div>
<div style="border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in">
<p class="MsoNormal"><b>From:</b> Laue, Thomas <Tom.Laue@unh.edu> <br>
<b>Sent:</b> Sunday, May 2, 2021 2:07 PM<br>
<b>To:</b> Borries Demeler <demeler@gmail.com>; Jeff Arthur <jarthur@kbibiopharma.com><br>
<b>Cc:</b> rasmb@list.rasmb.org<br>
<b>Subject:</b> RE: [RASMB] Optima AUC Interference optics question<o:p></o:p></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<div>
<p class="MsoNormal" style="background:#D5EAFF"><span style="font-size:12.0pt;font-family:"Cambria",serif;color:red">This is an EXTERNAL EMAIL. DO NOT click links or open attachments unless you recognize the sender AND know the content is safe. If you think
this email is malicious please click on the Phish Alert button above. </span><o:p></o:p></p>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<div>
<p class="MsoNormal"><span style="font-size:14.0pt;font-family:"Times New Roman",serif">Hi-<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:14.0pt;font-family:"Times New Roman",serif">I agree with Bo- there is some piece of crud ~6.5 cm in your optics. I would check out the most exposed places first- with the lens (or lens cover) at the bottom of the
chamber. The remaining optics should be sealed… if they are open for some reason, the horizontal surfaces on the lower track would be my next place to look. A bit of history, the lower track originally was hermetically sealed to allow a slight vacuum to be
pulled to eliminate air convection from contributing to the fringe displacement. As it turned out, the ‘rocking’ of the bottom chamber lens during a heating/cooling cycle swamped the fringe disturbance from convection.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:14.0pt;font-family:"Times New Roman",serif">Cheers-<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:14.0pt;font-family:"Times New Roman",serif">Tom
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:14.0pt;font-family:"Times New Roman",serif"><o:p> </o:p></span></p>
<div>
<div style="border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in">
<p class="MsoNormal"><b>From:</b> RASMB <<a href="mailto:rasmb-bounces@list.rasmb.org">rasmb-bounces@list.rasmb.org</a>>
<b>On Behalf Of </b>Borries Demeler<br>
<b>Sent:</b> Saturday, May 1, 2021 2:59 PM<br>
<b>To:</b> Jeff Arthur <<a href="mailto:jarthur@kbibiopharma.com">jarthur@kbibiopharma.com</a>><br>
<b>Cc:</b> <a href="mailto:rasmb@list.rasmb.org">rasmb@list.rasmb.org</a><br>
<b>Subject:</b> Re: [RASMB] Optima AUC Interference optics question<o:p></o:p></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal" style="margin-bottom:12.0pt"><strong><span style="font-family:"Calibri",sans-serif;color:red">Caution - External Email</span></strong><o:p></o:p></p>
<div>
<div>
<p class="MsoNormal">Take a look at the original images, there is probably a black spot at this position somewhere in the image. My guess is you have dirt in the optics or dead pixels in the camera. This noise offsets the Fourier transform and you get random
fringe shifts. If the noise streaks through the entire image at the same radial position it is likely just dirt. Carefully inspect the top of the camera and see if you can see anything stuck on the top and remove it carefully. Anything below that should be
addressed by Beckman service. <o:p></o:p></p>
<div>
<p class="MsoNormal">-Borries <o:p></o:p></p>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
</div>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<div>
<div>
<p class="MsoNormal">On Fri, Apr 30, 2021 at 9:46 PM Jeff Arthur <<a href="mailto:jarthur@kbibiopharma.com">jarthur@kbibiopharma.com</a>> wrote:<o:p></o:p></p>
</div>
<blockquote style="border:none;border-left:solid #CCCCCC 1.0pt;padding:0in 0in 0in 6.0pt;margin-left:4.8pt;margin-top:5.0pt;margin-right:0in;margin-bottom:5.0pt">
<div>
<div>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Hi Everyone,<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">I am having an issue with the interference optics on my Optima and I am hoping to get any advice on how to troubleshoot. The issue is screenshot below, which shows two successive
scans on each of two cells. My last couple runs have a blip at ~6.5 cm. It is present in every cell, so I assume that it is instrument related. At radial positions lower than the blip, the fringe shift is inconsistent between successive scans, which is creating
difficulties in analyzing the results, even if the region immediately surrounding 6.5 cm is excluded from the raw data. It’s probably coincidental, but I also noticed that the revolution counter decreased significantly immediately before this issue cropped
up. Before the issue it was ~550,000, and not it is ~450,000.<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Thanks for the help!<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Jeff<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><o:p> </o:p></p>
</div>
</div>
<p class="MsoNormal">_______________________________________________<br>
RASMB mailing list<br>
<a href="mailto:RASMB@list.rasmb.org">RASMB@list.rasmb.org</a><br>
<a href="https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flist.rasmb.org%2Flistinfo.cgi%2Frasmb-rasmb.org&data=04%7C01%7Cjarthur%40kbibiopharma.com%7C37785deaa33e4507a70008d90da5d542%7C6fe0d4cd6bf54b309cb1853589ea719f%7C0%7C0%7C637555828180561206%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=IIb10sCJn9ga1YPRhlH0W74iqUUj5dpzL8IoCNzG0Wk%3D&reserved=0">http://list.rasmb.org/listinfo.cgi/rasmb-rasmb.org</a><o:p></o:p></p>
</blockquote>
</div>
</div>
</div>
</div>
</body>
</html>