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<p>Hi Alessandro,</p>
<p>It sounds like the ultracentrifuge has hung. When this happens
one could shut down everything. Recycle the power and start over.<br>
When you do this use the stop button on the 'fuge. When the rotor
comes to a rest wait 30 minutes to allow the diffusion pump to
cool*. <br>
You do not have to vent the chamber for a short power cycle on the
fuge. Turn off the fuge, (after the 30 minute cooling period), for
about 10 seconds.<br>
Then turn it back on. It should not vent the chamber. It will
restart the vacuum pump and continue to pull down the chamber.<br>
</p>
<p>Cycle the power on the PC and launch you AUC app. (cold start the
PC by shutting down and turning the power off)</p>
<p>If the fuge won't respond to the stop button one could cycle the
fuge power while it's running at 3k as a last resort. This IS NOT
recommended with a hot diff pump.</p>
<p>The bottom line is the fuge has NVRAM (non-vol-ital ram) on the
instrument control board, and the CRT control board. These "chips"
have a battery on board the chip to hold memory when the power is
off. This battery is a Lithium battery with a life span of 10
years or less (sometimes more). If your machine is more than 10
years old, and these chips have not been replace, it is likely
your chips are getting bad (the internal batteries are dying). You
can buy these chips from Beckman Coulter however they are
expensive from them. They can be purchased from electronic part
suppliers for around $30 each US. <br>
If you have a qualified electronic person they could replace them.
These chips are in sockets and are intended to be replace.<br>
There is a clock chip on the CRT control board that needs to be
replace for the same reason, a dead battery. This resolves itself
as the date on the fuge appearing as ??/??/??. The time is also
incorrect on the fuge. (not the PC) The clock chip can be more
complicated as sometimes it is not in a socket.<br>
</p>
<p>If you are interested in doing this, I can help you. If you don't
feel comfortable doing this, call your Beckman Coulter service
rep. <br>
</p>
<p>Overspeed disks are expensive and would likely not do anything
for this problem.<br>
<br>
John Gunther<br>
Rocky Mtn SIMS, LLC</p>
<p><br>
</p>
<p>* allowing the diff pump to cool will reduce the possibility of
free vaporous diff pump oil condensing on the optical components.<br>
</p>
<div class="moz-cite-prefix">On 2/11/2020 7:24 AM, Marius Schmid
wrote: <br>
</div>
<blockquote type="cite"
cite="mid:1306421279.2446218.1581431064128@mail.yahoo.com">
<meta http-equiv="content-type" content="text/html; charset=UTF-8">
<div class="ydp7ef98f6ayahoo-style-wrap"
style="font-family:Helvetica Neue, Helvetica, Arial,
sans-serif;font-size:16px;">
<div dir="ltr" data-setdir="false">Dear Alessandro, <br>
</div>
<div dir="ltr" data-setdir="false"><br>
</div>
<div dir="ltr" data-setdir="false">If I remember correctly, I
might have seen an error like this in the past. But I am not
entirely sure if it was caused by the overspeed disc of the
rotor or an malfunction of one of the control boards. Did you
try to exchange the overspeed disc of the rotor? There might
be a problem with the magnets on the overspeed disc and the
hall sensor. <br>
</div>
<div dir="ltr" data-setdir="false"><br>
</div>
<div dir="ltr" data-setdir="false">best wishes, <br>
</div>
<div dir="ltr" data-setdir="false">Marius<br>
</div>
<div><br>
</div>
</div>
<div id="yahoo_quoted_1539196366" class="yahoo_quoted">
<div style="font-family:'Helvetica Neue', Helvetica, Arial,
sans-serif;font-size:13px;color:#26282a;">
<div> Am Dienstag, 11. Februar 2020, 14:40:59 MEZ hat
Alessandro S. Nascimento <a class="moz-txt-link-rfc2396E" href="mailto:asnascimento@ifsc.usp.br"><asnascimento@ifsc.usp.br></a>
Folgendes geschrieben: </div>
<div><br>
</div>
<div><br>
</div>
<div>
<div id="yiv2288601492">
<div dir="ltr">
<div>Dear RASMB members,</div>
<div><br>
</div>
<div>we are having a problem with our XL-A. I've
searched in the list and the possible solutions I
could find did not solve the problem.</div>
<div><br>
</div>
<div>Once the experiment is set up, with stabilized
temperature and vacuum (0 micron) running at 3000 rpm
the instrument status keeps as "waiting for speed"
while the instrument is already stabilized @ 3000 rpm.
<br>
</div>
<div><br>
</div>
<div>We tried to rapidly remove the white jumper and
plug it again and also tried to setup a non-zero
velocity with the door open (forcing a drive
diagnostic). Those suggestions appeared in the list in
the past. None of these could restore the sync between
the instrument and the software.</div>
<div><br>
</div>
<div>Any clues on what could be going on?</div>
<div><br>
</div>
<div>I thank in advance,</div>
<div><br>
</div>
<div>-Alessandro<br>
</div>
<div><br>
-- <br>
<div dir="ltr" class="yiv2288601492gmail_signature">
<div dir="ltr">
<div>
<div dir="ltr"><span
style="color:rgb(33,33,33);font-family:Helvetica,
Arial, sans-serif;font-size:13px;">********************************************************</span>
<div
style="color:rgb(33,33,33);font-family:Helvetica,
Arial, sans-serif;font-size:13px;">Alessandro
S. Nascimento</div>
<div
style="color:rgb(33,33,33);font-family:Helvetica,
Arial, sans-serif;font-size:13px;">Professor</div>
<div
style="color:rgb(33,33,33);font-family:Helvetica,
Arial, sans-serif;font-size:13px;">São
Carlos Institute of Physics (IFSC)</div>
<div
style="color:rgb(33,33,33);font-family:Helvetica,
Arial, sans-serif;font-size:13px;">University
of São Paulo (USP) - Brazil<br>
</div>
<div
style="color:rgb(33,33,33);font-family:Helvetica,
Arial, sans-serif;font-size:13px;">E-mail: <a
rel="nofollow"
ymailto="mailto:asnascimento@ifsc.usp.br"
target="_blank"
href="mailto:asnascimento@ifsc.usp.br"
moz-do-not-send="true">asnascimento@ifsc.usp.br</a></div>
<div
style="color:rgb(33,33,33);font-family:Helvetica,
Arial, sans-serif;font-size:13px;">Phone:
+55-16-3373-8709</div>
<div
style="color:rgb(33,33,33);font-family:Helvetica,
Arial, sans-serif;font-size:13px;">********************************************************</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
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<pre class="moz-quote-pre" wrap="">_______________________________________________
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