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We also run AUC of proteins in serum using commercial human and
murine serum (ref attached). Commercial human serum is usually
tested for infectious material before selling but it might need
higher level of bio-safety if you use serum from patients. <br>
As for serum from health volunteer, it depends on the guideline of
the organization.<br>
<br>
I guess AUC has filter not HEPA but "BioSafe optional dual PALL
Pharmaceutical-Grade Sterilizing Filters" which is installed for
preparative UC at the vacuum exhaust. <br>
<br>
Best,<br>
Susumu<br>
<br>
<blockquote type="cite"
cite="mid:4DB59BBDA90615479BF3E808FE5984FE01967F4986@NTExMbx8.ntummc.umsmed.edu">
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<p class="MsoNormal"><b>From:</b> RASMB
[<a class="moz-txt-link-freetext" href="mailto:rasmb-bounces@list.rasmb.org">mailto:rasmb-bounces@list.rasmb.org</a>] <b>
On Behalf Of </b>John Sumida<br>
<b>Sent:</b> Wednesday, September 26, 2018 5:00 PM<br>
<b>To:</b> RASMB <a class="moz-txt-link-rfc2396E" href="mailto:rasmb@list.rasmb.org"><rasmb@list.rasmb.org></a><br>
<b>Subject:</b> [EXTERNAL] [RASMB] Serum and EH&S<o:p></o:p></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Dear RASMB,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I apologize that this is not a scientific
question, but this is, perhaps, a question that has come up
for some on the list and I would be curious to hear how it was
handled.
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">We periodically field requests to spin
human serum solutions, which invariably is a health &
safety problem due to the potential for a cell leak and the
subsequent aerosolization of a potentially hazardous material
into the lab environment.
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">As an academic lab, our resources and
institutional motivation in engineering a space specific for
this application are limited. Thus, one suggestion was to use
third party testing of samples being submitted for analysis.
However this is costly and most users have abandoned their
efforts to perform these measurements as a result.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I would be curious to know if third party
testing of potentially infectious materials such as human
serum is commonly used within an EH&S setting, or if it is
possible to place a HEPA filter in the vacuum exhaust if the
instrument uses a TMP?<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thank you in advance for any information
you may be able to offer.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Best regards<o:p></o:p></p>
<p class="MsoNormal">John Sumida<o:p></o:p></p>
<p class="MsoNormal">University of Washington.<o:p></o:p></p>
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