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<div class="moz-cite-prefix">Dear John,<br>
<br>
Yes, our AUC is placed in BS-L1 level room.<br>
<br>
Best regards,<br>
Susumu<br>
<br>
<br>
<br>
<br>
On 2018/09/28 5:39, John Sumida wrote:<br>
</div>
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<div class="WordSection1">
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Dear
Susumu,<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Thank
you for your response. Interesting that your facility does
accept third party testing of human serum samples. I will
be meeting with our EH&S group next week and this
information together with John’s will be helpful. I assume
that you are running your AUC under standard BS-L1
conditions, and that it is sufficient for you to show that
your samples have been tested for blood borne pathogens?<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Once
again, thank you for taking the time to respond to my
question.<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Best
regards,<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">John
Sumida<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">University
of Washington.<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<div>
<div style="border:none;border-top:solid #E1E1E1
1.0pt;padding:3.0pt 0in 0in 0in">
<p class="MsoNormal"><b><span
style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:windowtext">From:</span></b><span
style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:windowtext">
RASMB [<a class="moz-txt-link-freetext" href="mailto:rasmb-bounces@list.rasmb.org">mailto:rasmb-bounces@list.rasmb.org</a>] <b>On
Behalf Of </b>Susumu UCHIYAMA<br>
<b>Sent:</b> Thursday, September 27, 2018 12:34 PM<br>
<b>To:</b> <a class="moz-txt-link-abbreviated" href="mailto:rasmb@list.rasmb.org">rasmb@list.rasmb.org</a><br>
<b>Subject:</b> Re: [RASMB] [EXTERNAL] Serum and
EH&S<o:p></o:p></span></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">We also run AUC of proteins in serum using
commercial human and murine serum (ref attached). Commercial
human serum is usually tested for infectious material before
selling but it might need higher level of bio-safety if you
use serum from patients. <br>
As for serum from health volunteer, it depends on the
guideline of the organization.<br>
<br>
I guess AUC has filter not HEPA but "BioSafe optional dual
PALL Pharmaceutical-Grade Sterilizing Filters" which is
installed for preparative UC at the vacuum exhaust. <br>
<br>
Best,<br>
Susumu<br>
<br>
<br>
<o:p></o:p></p>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<div>
<div>
<div style="border:none;border-top:solid #E1E1E1
1.0pt;padding:3.0pt 0in 0in 0in">
<p class="MsoNormal"
style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><b>From:</b>
RASMB [<a href="mailto:rasmb-bounces@list.rasmb.org"
moz-do-not-send="true">mailto:rasmb-bounces@list.rasmb.org</a>]
<b>On Behalf Of </b>John Sumida<br>
<b>Sent:</b> Wednesday, September 26, 2018 5:00 PM<br>
<b>To:</b> RASMB <a
href="mailto:rasmb@list.rasmb.org"
moz-do-not-send="true"><rasmb@list.rasmb.org></a><br>
<b>Subject:</b> [EXTERNAL] [RASMB] Serum and EH&S<o:p></o:p></p>
</div>
</div>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Dear
RASMB,<o:p></o:p></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">I
apologize that this is not a scientific question, but this
is, perhaps, a question that has come up for some on the
list and I would be curious to hear how it was handled. <o:p></o:p></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">We
periodically field requests to spin human serum solutions,
which invariably is a health & safety problem due to
the potential for a cell leak and the subsequent
aerosolization of a potentially hazardous material into
the lab environment. <o:p></o:p></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">As
an academic lab, our resources and institutional
motivation in engineering a space specific for this
application are limited. Thus, one suggestion was to use
third party testing of samples being submitted for
analysis. However this is costly and most users have
abandoned their efforts to perform these measurements as a
result.<o:p></o:p></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">I
would be curious to know if third party testing of
potentially infectious materials such as human serum is
commonly used within an EH&S setting, or if it is
possible to place a HEPA filter in the vacuum exhaust if
the instrument uses a TMP?<o:p></o:p></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Thank
you in advance for any information you may be able to
offer.<o:p></o:p></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Best
regards<o:p></o:p></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">John
Sumida<o:p></o:p></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">University
of Washington.<o:p></o:p></p>
</div>
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<o:p></o:p></p>
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