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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link="#0563C1" vlink="#954F72"><div class=WordSection1><p class=MsoNormal><span style='color:#1F497D'>Dear John,<o:p></o:p></span></p><p class=MsoNormal><span style='color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='color:#1F497D'>Thank you for your response to my question. Interesting that your institution accepts third party testing of human serum samples. Ours does not currently have any formal policy in place but has taken the position that it is not possible to test for all potential blood borne pathogens.<o:p></o:p></span></p><p class=MsoNormal><span style='color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='color:#1F497D'>With the older diffusion pump based instrument, I think doing these measurements was more feasible since one did not have to worry about damaging the TMP?<o:p></o:p></span></p><p class=MsoNormal><span style='color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='color:#1F497D'>Do you have any recommended literature to point me at with respect to measuring the hydrodynamic scaling constant (ks) or the second viral coefficient (BMI)? Do you do the latter by DLS or Sed Eq?<o:p></o:p></span></p><p class=MsoNormal><span style='color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='color:#1F497D'>Thanks again for your response, and I hope that next time I post on this topic I will have more interesting questions and some actual data to query the group about.<o:p></o:p></span></p><p class=MsoNormal><span style='color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='color:#1F497D'>Best regards,<o:p></o:p></span></p><p class=MsoNormal><span style='color:#1F497D'>John Sumida<o:p></o:p></span></p><p class=MsoNormal><span style='color:#1F497D'>University of Washington<o:p></o:p></span></p><div><div style='border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in'><p class=MsoNormal><b>From:</b> RASMB [mailto:rasmb-bounces@list.rasmb.org] <b>On Behalf Of </b>John J. Correia<br><b>Sent:</b> Thursday, September 27, 2018 11:58 AM<br><b>To:</b> John Sumida <jpsumida@uw.edu>; RASMB <rasmb@list.rasmb.org><br><b>Subject:</b> Re: [RASMB] [EXTERNAL] Serum and EH&S<o:p></o:p></p></div></div><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><span style='color:#1F497D'>John, my lab has been doing human serum proteins and human serum runs without concerns (see attached for relevant studies). When you buy human serum it’s been tested for infectious material. The normal precautions of wearing gloves, a face mask, and then cleaning cell components are encouraged. Interpretation of the data is a bit harder since you must include ks and BM1 cross term values and thus know something about the serum composition and its interaction with your system of interest. This is part of the ongoing part of our work in this area.<o:p></o:p></span></p><p class=MsoNormal><a name="_MailEndCompose"></a><span style='color:#1F497D'><o:p> </o:p></span></p><div><div style='border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in'><p class=MsoNormal><b>From:</b> RASMB [<a href="mailto:rasmb-bounces@list.rasmb.org">mailto:rasmb-bounces@list.rasmb.org</a>] <b>On Behalf Of </b>John Sumida<br><b>Sent:</b> Wednesday, September 26, 2018 5:00 PM<br><b>To:</b> RASMB <<a href="mailto:rasmb@list.rasmb.org">rasmb@list.rasmb.org</a>><br><b>Subject:</b> [EXTERNAL] [RASMB] Serum and EH&S<o:p></o:p></p></div></div><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Dear RASMB,<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>I apologize that this is not a scientific question, but this is, perhaps, a question that has come up for some on the list and I would be curious to hear how it was handled. <o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>We periodically field requests to spin human serum solutions, which invariably is a health & safety problem due to the potential for a cell leak and the subsequent aerosolization of a potentially hazardous material into the lab environment. <o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>As an academic lab, our resources and institutional motivation in engineering a space specific for this application are limited. Thus, one suggestion was to use third party testing of samples being submitted for analysis. However this is costly and most users have abandoned their efforts to perform these measurements as a result.<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>I would be curious to know if third party testing of potentially infectious materials such as human serum is commonly used within an EH&S setting, or if it is possible to place a HEPA filter in the vacuum exhaust if the instrument uses a TMP?<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Thank you in advance for any information you may be able to offer.<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Best regards<o:p></o:p></p><p class=MsoNormal>John Sumida<o:p></o:p></p><p class=MsoNormal>University of Washington.<o:p></o:p></p><p class=MsoNormal><span style='font-size:12.0pt;font-family:"Times New Roman",serif'>Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. <o:p></o:p></span></p></div></body></html>