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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link="#0563C1" vlink="#954F72"><div class=WordSection1><p class=MsoNormal>Dear RASMB,<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>I’ve been reviewing some posts on the list regarding the use of various organic solvents in the AUC. Most of the posts appear to refer specifically to equilibrium experiments as opposed to velocity experiments, some posts recommend the Kel-F centerpieces over the Charcoal-epon, some cite a small explosion hazard in the use of flammable organics.<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>As there appears to be a wide variation in the posted recommendations I would like to ask two questions:<o:p></o:p></p><p class=MsoListParagraph style='text-indent:-.25in;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='mso-list:Ignore'>1.<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>Can the vacuum be exhausted into a fume hood in the case of an instrument with a turbo-molecular pump.<o:p></o:p></p><p class=MsoListParagraph style='text-indent:-.25in;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='mso-list:Ignore'>2.<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>Since many organic solvents are compressible (ie. chloroform, dichloromethane, toluene) and can thus generate a density difference in the cell, how does one account for these affects in a velocity experiment.<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>As these questions are broad and I know your time is precious, any pertinent literature references that list members might point me at would be very helpful to me. Additionally any operational/safety concerns list members might have compiled from experience working in non-aqueous solvents would also be appreciated.<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Best regards and thank you for your kind attention.<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>John Sumida<o:p></o:p></p><p class=MsoNormal>Molecular Analysis Facility<o:p></o:p></p><p class=MsoNormal>University of Washington<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p></div></body></html>