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<p class="MsoNormal">Dear RASMBers-<o:p></o:p></p>
<p class="MsoNormal">Will is entirely correct that the best way to align the cells is using the channel walls, which should fall along a radius. Spin Analytical has developed a commercial optical alignment tool that uses the same principle as outlined in the
paper. Contact Kiara at Spin (<a href="mailto:kiara.jordon@spinanalytical.com">kiara.jordon@spinanalytical.com</a>) for more information.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I do want to point out that the Spin CAT tool is designed to work with Spin cell housings. The accuracy and precision of the cell alignment is impacted directly by the accuracy of the of the cell-housing slots used for alignment. Beckman
housings have neither the requisite precision (housing-to-housing reproducibility) or accuracy (the slots must be perpendicular to the central cell radius). Hence, the added precision of Spin CAT alignment described in the paper is expected. Unfortunately,
so is the reduced accuracy and precision from the Beckman cell housings.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Best wishes,<o:p></o:p></p>
<p class="MsoNormal">Tom<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><b>From:</b> RASMB [mailto:rasmb-bounces@list.rasmb.org] <b>
On Behalf Of </b>William F Weiss IV<br>
<b>Sent:</b> Wednesday, May 24, 2017 8:39 AM<br>
<b>To:</b> rasmb@list.rasmb.org<br>
<b>Subject:</b> [RASMB] Optical alignment for AUC<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Fellow RASMBers,<o:p></o:p></p>
<p class="MsoNormal">If you have ever struggled with alignment problems when using SV-AUC to quantify levels of soluble aggregates, then you might find this interesting:<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><b>An optical alignment system improves precision of soluble aggregate quantitation by sedimentation velocity analytical ultracentrifugation<o:p></o:p></b></p>
<p class="MsoNormal">ABSTRACT:<o:p></o:p></p>
<p class="MsoNormal">Appropriate characterization of soluble aggregates is an important aspect of biologics development and manufacturing, and sedimentation velocity analytical ultracentrifugation (SV-AUC) is often used an orthogonal technique to size-exclusion
chromatography (SEC) for this purpose. Precise quantification of low levels of soluble aggregates by SV-AUC can be adversely impacted by improper cell alignment. This report describes the development of an optical system capable of quantifying cell alignment
that affords a substantial improvement compared to historical approaches.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">The journal provides temporary free access to the article:<o:p></o:p></p>
<p class="MsoPlainText"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__authors.elsevier.com_a_1V5iolbALfkA&d=DwMFAg&c=c6MrceVCY5m5A_KAUkrdoA&r=hAcbVT23dt1uq6AGxObZKg&m=Kk2luH-2oREpqPNOyNI6EEokysKIf0nWeyqvnFM5Pj4&s=Qj5_pRSrgJeBHb6pT4zaNLDQSS4R_RCEQRyhiXFbSa8&e=">https://authors.elsevier.com/a/1V5iolbALfkA</a><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">We look forward to future developments in the field. Thanks.<o:p></o:p></p>
<p class="MsoNormal">-Will<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal" style="text-autospace:none"><b><span style="font-family:"Arial",sans-serif;color:#867870">William F. Weiss IV, Ph.D.<o:p></o:p></span></b></p>
<p class="MsoNormal" style="text-autospace:none"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#82786F">Principal Research Scientist<o:p></o:p></span></p>
<p class="MsoNormal" style="text-autospace:none"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#82786F">Biophysical Characterization Group Leader<o:p></o:p></span></p>
<p class="MsoNormal" style="text-autospace:none"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#82786F">Biopharmaceutical Research and Development</span><span style="font-size:10.0pt;font-family:ArialMT;color:#867870"><o:p></o:p></span></p>
<p class="MsoNormal" style="text-autospace:none"><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#D52B1E">Eli Lilly and Company<o:p></o:p></span></b></p>
<p class="MsoNormal" style="text-autospace:none"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#82786F">317.433.8260 (office) | 317.209.4748 (mobile)<o:p></o:p></span></p>
<p class="MsoNormal" style="text-autospace:none"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#82786F"><a href="mailto:weisswf@lilly.com"><span style="color:blue">weisswf@lilly.com</span></a> |
<a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.lilly.com&d=DwMFAg&c=c6MrceVCY5m5A_KAUkrdoA&r=hAcbVT23dt1uq6AGxObZKg&m=Kk2luH-2oREpqPNOyNI6EEokysKIf0nWeyqvnFM5Pj4&s=evHR2lgbz2cy8Nj_nZvLv_7kZzkewD7mZEKh54JHJGI&e=">
<span style="color:blue">www.lilly.com</span></a><o:p></o:p></span></p>
<p class="MsoNormal" style="text-autospace:none"><span style="font-size:10.0pt;font-family:ArialMT;color:#82786F"><o:p> </o:p></span></p>
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