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<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Dear all,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">The SensiQ is now again on the market and sold by ForteBio/Pall.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">See:
<a href="http://www.fortebio.com/news_details.html?id=49">http://www.fortebio.com/news_details.html?id=49</a>
<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">I evaluated the Taylor dispersion gradient set-up a few years ago, but apparently, it has been significantly improved
now, and would deserve to be re-tested.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Best,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Patrick<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Patrick ENGLAND<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Chair of the ARBRE-MOBIEU European network<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">ARBRE : Association of Resources for Biophysical Research in Europe<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">MOBIEU : Molecular Biophysics in Europe<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Web-site :
</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><a href="http://www.arbre-mobieu.eu/"><span lang="EN-US" style="color:#0563C1">http://www.arbre-mobieu.eu</span></a></span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">
<span lang="EN-US"><o:p></o:p></span></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Mailing address :<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Biophysics of Macromolecules and their Interactions<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Institut Pasteur<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">25 rue du Docteur Roux<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">75015 Paris<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">France<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Tel :+33-1-44389474<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">e-mail :
<a href="mailto:patrick.england@pasteur.fr"><span style="color:#0563C1">patrick.england@pasteur.fr</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Web-site :
</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><a href="https://research.pasteur.fr/en/team/biophysics/"><span lang="EN-US" style="color:#0563C1">http://www.pasteur.fr/biophysics/</span></a></span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">
<span lang="EN-US"><o:p></o:p></span></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">De :</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> RASMB [mailto:rasmb-bounces@list.rasmb.org]
<b>De la part de</b> Huber, Sylwia<br>
<b>Envoyé :</b> jeudi 2 mars 2017 09:21<br>
<b>À :</b> John Sumida <jpsumida@uw.edu><br>
<b>Cc :</b> RASMB <rasmb@list.rasmb.org><br>
<b>Objet :</b> Re: [RASMB] Taylor Dispersion SPR instrument<o:p></o:p></span></p>
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<p class="MsoNormal">Dear John,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">I am advanced user of SPR technology and we work mainly with small molecules and DMSO solutions.<o:p></o:p></p>
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<p class="MsoNormal">Such instrument as a SensiQ may surely work in high throughput mode.<o:p></o:p></p>
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<p class="MsoNormal">I am, however, not very convinced of the benefit of <span style="font-size:9.5pt">concentration gradient in a single injection.</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:9.5pt">We did some evaluation of SensiQ instrument and I think that after concentration gradient in a single injection, you have to make so or so an additional experiment to verify the results.</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:9.5pt">Some of the data we have collected during the evaluation were not properly evaluated by the software.</span><o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-size:9.5pt">Basically, after injecting of a single concentration in a standard SPR experiment, you get also the feeling where the affinity is. So my opinion is, that the benefit of the single injection is not so high as
promised and we have decided not to invest in a such instrument. I hope to help you, but please keep in mind it is my personal opinion.</span><o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-size:9.5pt">Kind regards,</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:9.5pt">Sylwia</span><o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-size:7.5pt">Mit freundlichen Grüssen/With Kind Regards<br>
<br>
<b>Dr. Sylwia Huber</b><br>
Principal Scientist, <i>Biophysics Team Head</i></span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:7.5pt">Roche Innovation Center Basel</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:7.5pt">F. Hoffmann-La Roche Ltd., Chemical Bi</span><span style="font-size:10.0pt">ology</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.0pt">Building 65/203, Grenzacherstrasse 124</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:7.5pt">CH-4070 Basel, Switzerland<br>
Phone: +41/61/6879507</span><o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">On Wed, Mar 1, 2017 at 10:25 PM, John Sumida <<a href="mailto:jpsumida@uw.edu" target="_blank">jpsumida@uw.edu</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US">Dear RASMB,<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US"> <o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US">This is not a question related to AUC.
<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US"> <o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US">I am evaluating an instrument that uses a dispersion cell to inject a concentration gradient in a single injection to perform an SPR experiment. This would be
similar to the SensiQ instrument that is no longer on the market. I would love to get a sense of the utility of operating an instrument such as this in core lab environment. I am particularly interested in assessing if this is truly a high throughput approach
and how robust the approach is with respected to small molecules which may need DMSO in solution for solubility.<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US"> <o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US">Thank you for your time and comments in advance
<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US"> <o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US">Best regards,<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US"><img border="0" width="119" height="48" id="m_-2766031919433588049Picture_x0020_1" src="cid:image001.jpg@01D29338.583D26E0"><o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US">John Sumida<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US">Molecular Analysis Facility<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US">University of Washington<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US"> <o:p></o:p></span></p>
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_______________________________________________<br>
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<a href="mailto:RASMB@list.rasmb.org">RASMB@list.rasmb.org</a><br>
<a href="http://list.rasmb.org/listinfo.cgi/rasmb-rasmb.org" target="_blank">http://list.rasmb.org/listinfo.cgi/rasmb-rasmb.org</a><o:p></o:p></p>
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