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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link="#0563C1" vlink="#954F72"><div class=WordSection1><p class=MsoPlainText style='margin-bottom:6.0pt'>Stephen, I think it is unlikely the <i>dn/dc</i> for your protein differs by more than 5% from your calculation. If there is a "major" discrepancy then calibration is not the issue. <o:p></o:p></p><p class=MsoPlainText style='margin-bottom:6.0pt'>John<o:p></o:p></p><p class=MsoPlainText><o:p> </o:p></p><p class=MsoPlainText>-----Original Message-----<br>From: RASMB [mailto:rasmb-bounces@list.rasmb.org] On Behalf Of Stephen McLaughlin<br>Sent: Thursday, March 17, 2016 5:08 AM<br>To: Mattia Rocco <mattia.rocco@hsanmartino.it><br>Cc: rasmb@rasmb.org<br>Subject: Re: [RASMB] dn/dc</p><p class=MsoPlainText><o:p> </o:p></p><p class=MsoPlainText>Dear Mattia, <o:p></o:p></p><p class=MsoPlainText><o:p> </o:p></p><p class=MsoPlainText>at the minute, it would be good to know if the dn/dc increases or decreases and roughy how much. There is a significant discrepancy between the molar mass measured by SEC-MALS and the expected mass from mass-spec. <o:p></o:p></p><p class=MsoPlainText><o:p> </o:p></p><p class=MsoPlainText>Best <o:p></o:p></p><p class=MsoPlainText><o:p> </o:p></p><p class=MsoPlainText>Stephen<o:p></o:p></p><p class=MsoPlainText><o:p> </o:p></p><p class=MsoPlainText><o:p> </o:p></p><p class=MsoPlainText><o:p> </o:p></p><p class=MsoPlainText>On 17 Mar 2016, at 09:53, Mattia Rocco wrote:<o:p></o:p></p><p class=MsoPlainText><o:p> </o:p></p><p class=MsoPlainText>> Dear Stephen,<o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> it likely will. But calculating a dn/dc is not that reliable anyway... and what's the level of precision you need, after all?<o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> Best - Mattia<o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> ________________________________<o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> From: RASMB on behalf of Stephen McLaughlin<o:p></o:p></p><p class=MsoPlainText>> Sent: Thu 3/17/2016 10:40 AM<o:p></o:p></p><p class=MsoPlainText>> To: <a href="mailto:rasmb@rasmb.org"><span style='color:windowtext;text-decoration:none'>rasmb@rasmb.org</span></a><o:p></o:p></p><p class=MsoPlainText>> Subject: [RASMB] dn/dc<o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> Dear all, <o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> I have a small three fingered Zn binding protein. Will the presence of Zn ions affect the refractive increment that can be caluculated from sequence?<o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> Best <o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> Stephen<o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> ______________________________<o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> Stephen McLaughlin, Ph.D.<o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> Biophysics<o:p></o:p></p><p class=MsoPlainText>> MRC Laboratory of Molecular Biology<o:p></o:p></p><p class=MsoPlainText>> Francis Crick Avenue<o:p></o:p></p><p class=MsoPlainText>> Cambridge Biomedical Campus<o:p></o:p></p><p class=MsoPlainText>> Cambridge, CB2 0QH<o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> Tel.: +44 (0) 1223 267891 (office)<o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> Email:stephenm@mrc-lmb.cam.ac.uk<o:p></o:p></p><p class=MsoPlainText>> _____________________________<o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText>> <o:p></o:p></p><p class=MsoPlainText><o:p> </o:p></p><p class=MsoPlainText>_______________________________________________<o:p></o:p></p><p class=MsoPlainText>RASMB mailing list<o:p></o:p></p><p class=MsoPlainText><a href="mailto:RASMB@list.rasmb.org"><span style='color:windowtext;text-decoration:none'>RASMB@list.rasmb.org</span></a><o:p></o:p></p><p class=MsoPlainText><a href="http://list.rasmb.org/listinfo.cgi/rasmb-rasmb.org"><span style='color:windowtext;text-decoration:none'>http://list.rasmb.org/listinfo.cgi/rasmb-rasmb.org</span></a><o:p></o:p></p></div></body></html>