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<p><span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; background-color: rgb(255, 255, 255);">Thanks everyone, I really appreciate the replies, I have several thing to try and think about :-)</span><br>
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<div style="margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Tahoma,sans-serif" size="2"><span style="font-size:10pt">- Ronald Toth</span></font></span></font></div>
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<div style="margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Tahoma,sans-serif" size="2"><span style="font-size:10pt">Research Associate</span></font></span></font></div>
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<div style="margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Tahoma,sans-serif" size="2"><span style="font-size:10pt">Macromolecule and Vaccine Stabilization Center</span></font></span></font></div>
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<div style="margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Tahoma,sans-serif" size="2"><span style="font-size:10pt">University of Kansas</span></font></span></font></div>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" color="#000000" style="font-size:11pt"><b>From:</b> RASMB <rasmb-bounces@list.rasmb.org> on behalf of Toth IV, Ronald T <Toth.Ronald@ku.edu><br>
<b>Sent:</b> Monday, December 14, 2015 4:15 PM<br>
<b>To:</b> rasmb@rasmb.org<br>
<b>Cc:</b> Kumru, Ozan S<br>
<b>Subject:</b> [RASMB] Possible for a complex to break apart from force of sedimentation?</font>
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<p>Hi all! We’re looking at a ~60 kDa protein that forms a ~ 1 MDa oligomeric disulfide linked complex. By SEC and DLS the sample is very monodisperse at about what we would expect the MDa complex to look like. However by AUC we see a very heterogeneous distribution
between 5s and 30s. I was wondering if anyone else had observed a system where a complex had broken apart from the force of sedimentation?<br>
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<p>Thanks in advance! <br>
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<div style="margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Tahoma,sans-serif" size="2"><span style="font-size:10pt">Research Associate</span></font></span></font></div>
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<div style="margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Tahoma,sans-serif" size="2"><span style="font-size:10pt">Macromolecule and Vaccine Stabilization Center</span></font></span></font></div>
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