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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link="#0563C1" vlink="#954F72"><div class=WordSection1><p class=MsoNormal style='margin-bottom:6.0pt'><span style='font-size:10.0pt;font-family:"Arial",sans-serif;color:blue'>David, I will speculate that you have a problem with the slit assembly. You might see something like that if the foil on the bottom side has come partially loose. Or perhaps there is some dirt or a burr causing the whole assembly to lift up at certain radial positions.<o:p></o:p></span></p><p class=MsoNormal style='margin-bottom:6.0pt'><span style='font-size:10.0pt;font-family:"Arial",sans-serif;color:blue'>I once had the foil come loose in a way that produced wild outlier scans, but only every once in while (a few per run). Intermittently the foil would actually catch on something (probably the ring on top of the PMT) and fold completely back on itself.<o:p></o:p></span></p><p class=MsoNormal style='margin-bottom:6.0pt'><span style='font-size:10.0pt;font-family:"Arial",sans-serif;color:blue'>And by the way, you should remind your people to fill the cells <u>much</u> more. You want the meniscus to be up near 5.9 cm, not at ~6.25 cm.<o:p></o:p></span></p><p class=MsoNormal style='margin-bottom:6.0pt'><span style='font-size:10.0pt;font-family:"Arial",sans-serif;color:blue'>John<o:p></o:p></span></p><div><div style='border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in'><p class=MsoNormal><b><span style='font-size:11.0pt;font-family:"Calibri",sans-serif'>From:</span></b><span style='font-size:11.0pt;font-family:"Calibri",sans-serif'> RASMB [mailto:rasmb-bounces@list.rasmb.org] <b>On Behalf Of </b>David Hayes<br><b>Sent:</b> Monday, October 19, 2015 11:14 AM<br><b>To:</b> RASMB <rasmb@rasmb.org><br><b>Subject:</b> [RASMB] Strange AUC noise<o:p></o:p></span></p></div></div><p class=MsoNormal><o:p> </o:p></p><div><div id="yui_3_16_0_1_1445277124881_2742"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>Hi all,<o:p></o:p></span></p></div><div id="yui_3_16_0_1_1445277124881_2742"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p></div><div id="yui_3_16_0_1_1445277124881_2751"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>We had some strange AUC noise in a recent experiment. In the pictures below from SEDFIT, the first two runs produced data as in the picture NNBekman1 (the IT guys name our instruments and they pruposely mispelled Beckman as Bekman years ago). A strange noise pattern showed up in the scans that were produced on day one. Then the sample was loaded into a new cell and run again on the same centrifuge producing the strange noise pattern in the picture called NNBekman1. the filled cell was taken and gently mixed and then run on another centrifuge Bekman17 and made a perfectly fine patter shown in picture Beckman17.<o:p></o:p></span></p></div><div id="yui_3_16_0_1_1445277124881_2751"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p></div><div id="yui_3_16_0_1_1445277124881_2751"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>We have seen the strange noise before on our NNBekman1 centrifuge, but this is the first time on NNBekman2.<o:p></o:p></span></p></div><div id="yui_3_16_0_1_1445277124881_2751"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p></div><div id="yui_3_16_0_1_1445277124881_2751"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>Has anyone seen noise like this? <o:p></o:p></span></p></div><div id="yui_3_16_0_1_1445277124881_2751"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>Any theories on the cause of the noise?<o:p></o:p></span></p></div><div id="yui_3_16_0_1_1445277124881_2751"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p></div><div id="yui_3_16_0_1_1445277124881_2751"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>It certainly looks like an optical artifact of some kind, but the time dependence is hard to explain.<o:p></o:p></span></p></div><div id="yui_3_16_0_1_1445277124881_2751"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p></div><div id="yui_3_16_0_1_1445277124881_2751"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>Kind Regards,<o:p></o:p></span></p></div><div id="yui_3_16_0_1_1445277124881_2751"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p></div><div id="yui_3_16_0_1_1445277124881_2751"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>David Hayes<o:p></o:p></span></p></div><div id="yui_3_16_0_1_1445277124881_2751"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p></div><div id="yui_3_16_0_1_1445277124881_2742"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p></div><div id="yui_3_16_0_1_1445277124881_2742"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p></div></div></div></body></html>