<html><head></head><body><div style="color:#000; background-color:#fff; font-family:HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif;font-size:16px"><div id="yui_3_16_0_1_1444929838612_3245"><span>Cool: a real explosion from an eye witness. </span></div><div id="yui_3_16_0_1_1444929838612_3245" dir="ltr"><span>Thanks for the information Tom.</span></div><div id="yui_3_16_0_1_1444929838612_3245" dir="ltr"><span id="yui_3_16_0_1_1444929838612_3390">Maybe Beckman is not being as over cautious as I originally thought. </span></div><div id="yui_3_16_0_1_1444929838612_3245" dir="ltr"><span><br></span></div><div id="yui_3_16_0_1_1444929838612_3245" dir="ltr"><span><br></span></div><div id="yui_3_16_0_1_1444929838612_3245" dir="ltr"><span>David</span></div><div id="yui_3_16_0_1_1444929838612_3245" dir="ltr"><span><br></span></div><br> <div style="font-family: HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif; font-size: 16px;" id="yui_3_16_0_1_1444929838612_3201"> <div style="font-family: HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif; font-size: 16px;" id="yui_3_16_0_1_1444929838612_3200"> <div dir="ltr" id="yui_3_16_0_1_1444929838612_3244"> <hr size="1" id="yui_3_16_0_1_1444929838612_3416"> <font size="2" face="Arial" id="yui_3_16_0_1_1444929838612_3243"> <b><span style="font-weight:bold;">From:</span></b> "Laue, Thomas" <Tom.Laue@unh.edu><br> <b><span style="font-weight: bold;">To:</span></b> David Hayes <drdavidbhayes@yahoo.com>; "jphilo@mailway.com" <jphilo@mailway.com>; RASMB <rasmb@rasmb.org> <br> <b><span style="font-weight: bold;">Sent:</span></b> Thursday, October 15, 2015 10:03 AM<br> <b><span style="font-weight: bold;">Subject:</span></b> RE: [RASMB] Volatile buffers in AUC question<br> </font> </div> <div class="y_msg_container" id="yui_3_16_0_1_1444929838612_3199"><br><div id="yiv7823142859"><style>#yiv7823142859 #yiv7823142859 --
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#yiv7823142859 </style><div id="yui_3_16_0_1_1444929838612_3198">
<div class="yiv7823142859WordSection1" id="yui_3_16_0_1_1444929838612_3197">
<div class="yiv7823142859MsoNormal" id="yui_3_16_0_1_1444929838612_3242"><span style="font-size:11.0pt;">Hi-</span></div>
<div class="yiv7823142859MsoNormal" id="yui_3_16_0_1_1444929838612_3240"><span style="font-size:11.0pt;" id="yui_3_16_0_1_1444929838612_3241">The small amount and relatively low flammability of IPA (I thought you might be running an India Pale Ale) insures that there is no danger. Flammability is
something to consider, however. </span></div>
<div class="yiv7823142859MsoNormal" id="yui_3_16_0_1_1444929838612_3196"><span style="font-size:11.0pt;" id="yui_3_16_0_1_1444929838612_3195"> True story- we were working on the interference optics many years ago and had used some red glyptal to seal some holes in the heat sink. I was in a hurry to
get it to dry, so turned on the pumps. Now glyptal has several very volatile and flammable solvents (mek, acetone, toluene), and, after a couple of minutes there was a pretty good sized explosion when a relay was thrown and the spark set off the gases. Thank
god no one was hurt by my stupidity, there was no fire, and the only damage was a blown fuse.
</span></div>
<div class="yiv7823142859MsoNormal"><span style="font-size:11.0pt;">Best wishes,</span></div>
<div class="yiv7823142859MsoNormal"><span style="font-size:11.0pt;">Tom</span></div>
<div class="yiv7823142859MsoNormal"><span style="font-size:11.0pt;"> </span></div>
<div class="qtdSeparateBR"><br><br></div><div class="yiv7823142859yqt9457637413" id="yiv7823142859yqt96443"><div id="yui_3_16_0_1_1444929838612_3421">
<div style="border:none;border-top:solid #B5C4DF 1.0pt;padding:3.0pt 0in 0in 0in;" id="yui_3_16_0_1_1444929838612_3420">
<div class="yiv7823142859MsoNormal" id="yui_3_16_0_1_1444929838612_3419"><b><span style="font-size:10.0pt;">From:</span></b><span style="font-size:10.0pt;" id="yui_3_16_0_1_1444929838612_3418"> RASMB [mailto:rasmb-bounces@list.rasmb.org]
<b>On Behalf Of </b>David Hayes<br clear="none">
<b>Sent:</b> Wednesday, October 14, 2015 1:46 PM<br clear="none">
<b>To:</b> jphilo@mailway.com; RASMB<br clear="none">
<b>Subject:</b> Re: [RASMB] Volatile buffers in AUC question</span></div>
</div>
</div>
<div class="yiv7823142859MsoNormal" id="yui_3_16_0_1_1444929838612_3422"> </div>
<div id="yui_3_16_0_1_1444929838612_3424">
<div id="yiv7823142859">
<div id="yiv7823142859yui_3_16_0_1_1444844725642_3366">
<div id="yiv7823142859yui_3_16_0_1_1444844725642_3365">
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4805">
<div class="yiv7823142859MsoNormal" style="background:white;"><span style="">Hi John,</span></div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4806">
<div class="yiv7823142859MsoNormal" style="background:white;" id="yui_3_16_0_1_1444929838612_3423"><span style=""> </span></div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4808">
<div class="yiv7823142859MsoNormal" style="background:white;" id="yui_3_16_0_1_1444929838612_3426"><span style="" id="yui_3_16_0_1_1444929838612_3425">I was told that the outlets of the vacuum pumps went right into the inside of the centrifuge: so the leak from the cell would be expelled as a vapor
into the chassis of the centrifuge.</span></div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4810">
<div class="yiv7823142859MsoNormal" style="background:white;" id="yui_3_16_0_1_1444929838612_3428"><span style="" id="yui_3_16_0_1_1444929838612_3427">Similarly, if a botox sample cell leaked, the toxin could go just about anywhere and they would have to throw away the centrifuge: (a second hand apocryphal
story from Allergan).</span></div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4810">
<div class="yiv7823142859MsoNormal" style="background:white;" id="yui_3_16_0_1_1444929838612_3429"><span style=""> </span></div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4810">
<div class="yiv7823142859MsoNormal" style="background:white;"><span style="">Kind Regards,</span></div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4810">
<div class="yiv7823142859MsoNormal" style="background:white;"><span style=""> </span></div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4810">
<div class="yiv7823142859MsoNormal" style="background:white;"><span style="">David</span></div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4810">
<div class="yiv7823142859MsoNormal" style="background:white;"><span style=""> </span></div>
</div>
<div class="yiv7823142859MsoNormal" style="background:white;"><span style=""> </span></div>
<div>
<div class="yiv7823142859MsoNormal" style="margin-bottom:12.0pt;background:white;"><span style=""> </span></div>
</div>
<div id="yiv7823142859yqt89152">
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4699">
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4698">
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4697">
<div align="center" class="yiv7823142859MsoNormal" style="text-align:center;background:white;">
<span style="">
</span><hr align="center" size="1" width="100%">
</div>
<div class="yiv7823142859MsoNormal" style="background:white;"><b><span style="font-size:10.0pt;">From:</span></b><span style="font-size:10.0pt;"> John Philo <<a rel="nofollow" shape="rect" ymailto="mailto:jphilo@mailway.com" target="_blank" href="mailto:jphilo@mailway.com">jphilo@mailway.com</a>><br clear="none">
<b>To:</b> 'David Hayes' <<a rel="nofollow" shape="rect" ymailto="mailto:drdavidbhayes@yahoo.com" target="_blank" href="mailto:drdavidbhayes@yahoo.com">drdavidbhayes@yahoo.com</a>>; 'RASMB' <<a rel="nofollow" shape="rect" ymailto="mailto:rasmb@rasmb.org" target="_blank" href="mailto:rasmb@rasmb.org">rasmb@rasmb.org</a>>
<br clear="none">
<b>Sent:</b> Wednesday, October 14, 2015 1:21 PM<br clear="none">
<b>Subject:</b> RE: [RASMB] Volatile buffers in AUC question</span><span style=""></span></div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4704">
<div class="yiv7823142859MsoNormal" style="background:white;"><span style=""> </span></div>
<div id="yiv7823142859">
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4703">
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4702">
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4701">
<div class="yiv7823142859MsoNormal" style="background:white;"><span style="font-size:10.0pt;">David, if it were leaking into vacuum (no oxygen), how could it burn? Am I missing something?</span><span style=""></span></div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4705">
<div class="yiv7823142859MsoNormal" style="background:white;"><span style="font-size:10.0pt;"> </span><span style=""></span></div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4706">
<div class="yiv7823142859MsoNormal" style="background:white;"><span style="font-size:10.0pt;">John</span><span style=""></span></div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4707">
<div class="yiv7823142859MsoNormal" style="background:white;"><span style="font-size:10.0pt;"> </span><span style=""></span></div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444844725642_3420">
<div class="yiv7823142859MsoNormal" style="margin-bottom:12.0pt;background:white;"><span style=""> </span></div>
</div>
<div id="yiv7823142859yqt97857">
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4709">
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4708" style="border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in;">
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4710">
<div class="yiv7823142859MsoNormal" style="background:white;"><b><span style="font-size:11.0pt;">From:</span></b><span style="font-size:11.0pt;"> RASMB [<a rel="nofollow" shape="rect" ymailto="mailto:rasmb-bounces@list.rasmb.org" target="_blank" href="mailto:rasmb-bounces@list.rasmb.org">mailto:rasmb-bounces@list.rasmb.org</a>]
<b>On Behalf Of </b>David Hayes<br clear="none">
<b>Sent:</b> Wednesday, October 14, 2015 9:59 AM<br clear="none">
<b>To:</b> RASMB <<a rel="nofollow" shape="rect" ymailto="mailto:rasmb@rasmb.org" target="_blank" href="mailto:rasmb@rasmb.org">rasmb@rasmb.org</a>><br clear="none">
<b>Subject:</b> [RASMB] Volatile buffers in AUC question</span><span style=""></span></div>
</div>
</div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4712">
<div class="yiv7823142859MsoNormal" style="background:white;"><span style=""> </span></div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4714">
<div id="yiv7823142859yui_3_16_0_1_1444841906429_2631">
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4713">
<div class="yiv7823142859MsoNormal" style="background:white;"><span style="">Hi all,</span></div>
</div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444841906429_2635">
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4715">
<div class="yiv7823142859MsoNormal" style="background:white;"><span style=""> </span></div>
</div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444841906429_2639">
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4716">
<div class="yiv7823142859MsoNormal" style="background:white;"><span style="">I have a sample where we want to run in a buffer with 10% IPA.</span></div>
</div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444841906429_2643">
<div id="yiv7823142859yui_3_16_0_1_1444844383306_4718">
<div class="yiv7823142859MsoNormal" style="background:white;"><span style="">In general, because of the possibility of ignition of flammable components if there was a cell leak, People at AUC workshops say not to run organic
solvents in the Beckman XLA/XLI instruments.</span></div>
</div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444841906429_2647">
<div id="yiv7823142859yui_3_16_0_1_1444844725642_3423">
<div class="yiv7823142859MsoNormal" style="background:white;"><span style="">Should I be worried about this buffer with 10% isopropyl alcohol?
</span></div>
</div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444841906429_2651">
<div id="yiv7823142859yui_3_16_0_1_1444844725642_3425">
<div class="yiv7823142859MsoNormal" style="background:white;"><span style="">Even if a cell leaked and 40 micro liters of IPA evaporated along with the aqueous components, how big of a flash would it be? Could it damage the
instrument?</span></div>
</div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444841906429_2655">
<div>
<div class="yiv7823142859MsoNormal" style="background:white;"><span style=""> </span></div>
</div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444841906429_2659">
<div>
<div class="yiv7823142859MsoNormal" style="background:white;"><span style="">I know that polymer chemists also run AUC and wonder if there is any actual experience with organic solvents and any verified incidents of cells leaking
and causing fire damage.</span></div>
</div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444841906429_2663">
<div>
<div class="yiv7823142859MsoNormal" style="background:white;"><span style=""> </span></div>
</div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444841906429_2667">
<div>
<div class="yiv7823142859MsoNormal" style="background:white;"><span style="">Kind Regards,</span></div>
</div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444841906429_2671">
<div>
<div class="yiv7823142859MsoNormal" style="background:white;"><span style=""> </span></div>
</div>
</div>
<div id="yiv7823142859yui_3_16_0_1_1444841906429_2675">
<div>
<div class="yiv7823142859MsoNormal" style="background:white;"><span style="">David Hayes</span></div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
<div class="yiv7823142859MsoNormal" style="margin-bottom:12.0pt;background:white;"><span style=""> </span></div>
</div>
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</div></div>
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