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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Hi-<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">The small amount and relatively low flammability of IPA (I thought you might be running an India Pale Ale) insures that there is no danger. Flammability is
something to consider, however. <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"> True story- we were working on the interference optics many years ago and had used some red glyptal to seal some holes in the heat sink. I was in a hurry to
get it to dry, so turned on the pumps. Now glyptal has several very volatile and flammable solvents (mek, acetone, toluene), and, after a couple of minutes there was a pretty good sized explosion when a relay was thrown and the spark set off the gases. Thank
god no one was hurt by my stupidity, there was no fire, and the only damage was a blown fuse.
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Best wishes,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Tom<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> RASMB [mailto:rasmb-bounces@list.rasmb.org]
<b>On Behalf Of </b>David Hayes<br>
<b>Sent:</b> Wednesday, October 14, 2015 1:46 PM<br>
<b>To:</b> jphilo@mailway.com; RASMB<br>
<b>Subject:</b> Re: [RASMB] Volatile buffers in AUC question<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black">Hi John,<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black">I was told that the outlets of the vacuum pumps went right into the inside of the centrifuge: so the leak from the cell would be expelled as a vapor
into the chassis of the centrifuge.<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black">Similarly, if a botox sample cell leaked, the toxin could go just about anywhere and they would have to throw away the centrifuge: (a second hand apocryphal
story from Allergan).<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black">Kind Regards,<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black">David<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><b><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black">From:</span></b><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black"> John Philo <<a href="mailto:jphilo@mailway.com">jphilo@mailway.com</a>><br>
<b>To:</b> 'David Hayes' <<a href="mailto:drdavidbhayes@yahoo.com">drdavidbhayes@yahoo.com</a>>; 'RASMB' <<a href="mailto:rasmb@rasmb.org">rasmb@rasmb.org</a>>
<br>
<b>Sent:</b> Wednesday, October 14, 2015 1:21 PM<br>
<b>Subject:</b> RE: [RASMB] Volatile buffers in AUC question</span><span style="font-family:"Helvetica","sans-serif";color:black"><o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Helvetica","sans-serif";color:black">David, if it were leaking into vacuum (no oxygen), how could it burn? Am I missing something?</span><span style="font-family:"Helvetica","sans-serif";color:black"><o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Helvetica","sans-serif";color:black"> </span><span style="font-family:"Helvetica","sans-serif";color:black"><o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Helvetica","sans-serif";color:black">John</span><span style="font-family:"Helvetica","sans-serif";color:black"><o:p></o:p></span></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt;background:white"><span style="font-family:"Helvetica","sans-serif";color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="background:white"><b><span style="font-size:11.0pt;font-family:"Helvetica","sans-serif";color:black">From:</span></b><span style="font-size:11.0pt;font-family:"Helvetica","sans-serif";color:black"> RASMB [<a href="mailto:rasmb-bounces@list.rasmb.org">mailto:rasmb-bounces@list.rasmb.org</a>]
<b>On Behalf Of </b>David Hayes<br>
<b>Sent:</b> Wednesday, October 14, 2015 9:59 AM<br>
<b>To:</b> RASMB <<a href="mailto:rasmb@rasmb.org">rasmb@rasmb.org</a>><br>
<b>Subject:</b> [RASMB] Volatile buffers in AUC question</span><span style="font-family:"Helvetica","sans-serif";color:black"><o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black"> <o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black">Hi all,<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black"> <o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black">I have a sample where we want to run in a buffer with 10% IPA.<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black">In general, because of the possibility of ignition of flammable components if there was a cell leak, People at AUC workshops say not to run organic
solvents in the Beckman XLA/XLI instruments.<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black">Should I be worried about this buffer with 10% isopropyl alcohol?
<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black">Even if a cell leaked and 40 micro liters of IPA evaporated along with the aqueous components, how big of a flash would it be? Could it damage the
instrument?<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black"> <o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black">I know that polymer chemists also run AUC and wonder if there is any actual experience with organic solvents and any verified incidents of cells leaking
and causing fire damage.<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black"> <o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black">Kind Regards,<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black"> <o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Helvetica","sans-serif";color:black">David Hayes<o:p></o:p></span></p>
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