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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link="#0563C1" vlink="#954F72"><div class=WordSection1><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial",sans-serif;color:blue'>David, if it were leaking into vacuum (no oxygen), how could it burn? Am I missing something?<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial",sans-serif;color:blue'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial",sans-serif;color:blue'>John<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial",sans-serif;color:blue'><o:p> </o:p></span></p><div><div style='border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in'><p class=MsoNormal><b><span style='font-size:11.0pt;font-family:"Calibri",sans-serif'>From:</span></b><span style='font-size:11.0pt;font-family:"Calibri",sans-serif'> RASMB [mailto:rasmb-bounces@list.rasmb.org] <b>On Behalf Of </b>David Hayes<br><b>Sent:</b> Wednesday, October 14, 2015 9:59 AM<br><b>To:</b> RASMB <rasmb@rasmb.org><br><b>Subject:</b> [RASMB] Volatile buffers in AUC question<o:p></o:p></span></p></div></div><p class=MsoNormal><o:p> </o:p></p><div><div id="yui_3_16_0_1_1444841906429_2631"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>Hi all,<o:p></o:p></span></p></div><div id="yui_3_16_0_1_1444841906429_2635"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'> <o:p></o:p></span></p></div><div id="yui_3_16_0_1_1444841906429_2639"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>I have a sample where we want to run in a buffer with 10% IPA.<o:p></o:p></span></p></div><div id="yui_3_16_0_1_1444841906429_2643"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>In general, because of the possibility of ignition of flammable components if there was a cell leak, People at AUC workshops say not to run organic solvents in the Beckman XLA/XLI instruments.<o:p></o:p></span></p></div><div id="yui_3_16_0_1_1444841906429_2647"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>Should I be worried about this buffer with 10% isopropyl alcohol? <o:p></o:p></span></p></div><div id="yui_3_16_0_1_1444841906429_2651"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>Even if a cell leaked and 40 micro liters of IPA evaporated along with the aqueous components, how big of a flash would it be? Could it damage the instrument?<o:p></o:p></span></p></div><div id="yui_3_16_0_1_1444841906429_2655"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'> <o:p></o:p></span></p></div><div id="yui_3_16_0_1_1444841906429_2659"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>I know that polymer chemists also run AUC and wonder if there is any actual experience with organic solvents and any verified incidents of cells leaking and causing fire damage.<o:p></o:p></span></p></div><div id="yui_3_16_0_1_1444841906429_2663"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'> <o:p></o:p></span></p></div><div id="yui_3_16_0_1_1444841906429_2667"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>Kind Regards,<o:p></o:p></span></p></div><div id="yui_3_16_0_1_1444841906429_2671"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'> <o:p></o:p></span></p></div><div id="yui_3_16_0_1_1444841906429_2675"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>David Hayes<o:p></o:p></span></p></div></div></div></body></html>