<html><head></head><body><div style="color:#000; background-color:#fff; font-family:HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif;font-size:16px"><div id="yui_3_16_0_1_1444852910225_6696"><span id="yui_3_16_0_1_1444852910225_7135">Thanks to Virgil and John for quick responses in good context. </span></div><div id="yui_3_16_0_1_1444852910225_6696"><span> </span></div><div id="yui_3_16_0_1_1444852910225_6696" dir="ltr"><span id="yui_3_16_0_1_1444852910225_7132">i usually take Beckman recommendations with a grain of salt: for instance, epon centerpieces are rated at 42,000 RPM, but I run them at 50,000 and 60,000 and they seem fine as long as there is not a cell leak. So if IPA does not have a warning from the over cautious Beckman, I feel very safe running 10% IPA buffer.</span></div><div id="yui_3_16_0_1_1444852910225_6696" dir="ltr"><span><br></span></div><div id="yui_3_16_0_1_1444852910225_6696" dir="ltr"><span>David Hayes</span></div><br> <div style="font-family: HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif; font-size: 16px;" id="yui_3_16_0_1_1444852910225_6680"> <div style="font-family: HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif; font-size: 16px;" id="yui_3_16_0_1_1444852910225_6679"> <div dir="ltr" id="yui_3_16_0_1_1444852910225_6678"> <hr size="1" id="yui_3_16_0_1_1444852910225_7131"> <font size="2" face="Arial" id="yui_3_16_0_1_1444852910225_6677"> <b><span style="font-weight:bold;">From:</span></b> Virgil Schirf <schirfv@biochem.uthscsa.edu><br> <b><span style="font-weight: bold;">To:</span></b> David Hayes <drdavidbhayes@yahoo.com> <br><b><span style="font-weight: bold;">Cc:</span></b> RASMB <rasmb@rasmb.org> <br> <b><span style="font-weight: bold;">Sent:</span></b> Wednesday, October 14, 2015 2:57 PM<br> <b><span style="font-weight: bold;">Subject:</span></b> Re: [RASMB] Volatile buffers in AUC question<br> </font> </div> <div class="y_msg_container" id="yui_3_16_0_1_1444852910225_6681"><br>David,<br clear="none"><br clear="none">According the Beckman compatibility guide:<br clear="none"><br clear="none">Flammability hazard. Not recommended for use in any type of centrifuge because vapors may be ignited by exposure to electrical contacts. Depending<br clear="none">on the centrifuge type, such exposure could occur either during normal centrifugation or under failure conditions.<br clear="none"><br clear="none">However, in the same guide isopropyl alcohol does not have that warning and is listed as satisfactory for both epon-charcoal and aluminum centerpieces.<br clear="none"><br clear="none">Best regards,<br clear="none"><br clear="none">Virgil<br clear="none"><br clear="none">> Hi all,<br clear="none">><br clear="none">><br clear="none">>  <br clear="none">> I have a samplewhere we want to run in a buffer with 10% IPA.<br clear="none">><br clear="none">> In general, becauseof the possibility of ignition of flammable components if there was a cellleak, People at AUC workshops say not to run organic<br clear="none">> solvents in the BeckmanXLA/XLI instruments.<br clear="none">><br clear="none">> Should I be worriedabout this buffer with 10% isopropyl alcohol? <br clear="none">><br clear="none">> Even if a cellleaked and 40 micro liters of IPA evaporated along with the aqueous components,how big of a flash would it be? Could itdamage the<br clear="none">> instrument?<br clear="none">><br clear="none">><br clear="none">>  <br clear="none">> I know that polymerchemists also run AUC and wonder if there is any actual experience with organicsolvents and any verified incidents of cells<br clear="none">> leaking and causing fire damage.<br clear="none">><br clear="none">><br clear="none">>  <br clear="none">> Kind Regards,<br clear="none">><br clear="none">><br clear="none">>  <div class="qtdSeparateBR"><br><br></div><div class="yqt4560930681" id="yqtfd06795"><br clear="none">> David Hayes</div><br clear="none">><br clear="none">> _______________________________________________<br clear="none">> RASMB mailing list<br clear="none">> <a shape="rect" ymailto="mailto:RASMB@list.rasmb.org" href="mailto:RASMB@list.rasmb.org">RASMB@list.rasmb.org</a><br clear="none">> <a shape="rect" href="http://list.rasmb.org/listinfo.cgi/rasmb-rasmb.org" target="_blank">http://list.rasmb.org/listinfo.cgi/rasmb-rasmb.org</a><br clear="none">><br clear="none"><br clear="none"><br clear="none">________________________________________________<br clear="none"><br clear="none">Virgil Schirf<br clear="none">UTHSCSA Center for Macromolecular Interactions<br clear="none">Room 421D, Mailcode 7760<br clear="none">7703 Floyd Curl Dr<br clear="none">San Antonio TX 78229-3900<br clear="none">Cell: (210) 382-3712<br clear="none">Email: <a shape="rect" ymailto="mailto:schirfv@biochem.uthscsa.edu" href="mailto:schirfv@biochem.uthscsa.edu">schirfv@biochem.uthscsa.edu</a><br clear="none">Web: <a shape="rect" href="http://cmmi.uthscsa.edu/" target="_blank">http://cmmi.uthscsa.edu</a><br clear="none">________________________________________________<br clear="none"><br clear="none">Confidentiality Notice: This transmission may contain confidential information that is legally privileged. The information is intended only for the<br clear="none">use of the recipient(s) named above. If you have received this transmission in error, please notify the sender immediately. You are hereby notified<br clear="none">that any disclosure, copying, distribution or the taking of any action in reliance on the contents other than the written intent of this<br clear="none">transmission is strictly prohibited.<div class="yqt4560930681" id="yqtfd53712"><br clear="none"><br clear="none"><br clear="none"></div><br><br></div> </div> </div> </div></body></html>