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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link=blue vlink=purple><div class=WordSection1><p class=MsoNormal style='margin-bottom:6.0pt'><span style='font-size:10.0pt;font-family:"Arial",sans-serif;color:blue'>Oh, you didn’t say you meant combustion after being expelled through the vacuum pump, so I thought you were talking about combustion inside the chamber. I tend to doubt a flash of the vapor coming out of the vacuum pump is very likely, or that the amount of energy from 40 microliters (< 1 cal I think) would do much anyway, but that is way outside my expertise.<o:p></o:p></span></p><p class=MsoNormal style='margin-bottom:6.0pt'><span style='font-size:10.0pt;font-family:"Arial",sans-serif;color:blue'>Regarding the Allergan rumor, yes they did build a fume hood around their centrifuge in case there was a leak of Botox, but I’m virtually certain they never trashed a centrifuge because there was one.<o:p></o:p></span></p><p class=MsoNormal style='margin-bottom:6.0pt'><span style='font-size:10.0pt;font-family:"Arial",sans-serif;color:blue'>John<o:p></o:p></span></p><div><div style='border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in'><p class=MsoNormal><b><span style='font-size:11.0pt;font-family:"Calibri",sans-serif'>From:</span></b><span style='font-size:11.0pt;font-family:"Calibri",sans-serif'> David Hayes [mailto:drdavidbhayes@yahoo.com] <br><b>Sent:</b> Wednesday, October 14, 2015 10:46 AM<br><b>To:</b> jphilo@mailway.com; RASMB <rasmb@rasmb.org><br><b>Subject:</b> Re: [RASMB] Volatile buffers in AUC question<o:p></o:p></span></p></div></div><p class=MsoNormal><o:p> </o:p></p><div><div id=yiv6391599244><div id="yui_3_16_0_1_1444844725642_3366"><div id="yui_3_16_0_1_1444844725642_3365"><div id="yiv6391599244yui_3_16_0_1_1444844383306_4805"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>Hi John,<o:p></o:p></span></p></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4806"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4808"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>I was told that the outlets of the vacuum pumps went right into the inside of the centrifuge: so the leak from the cell would be expelled as a vapor into the chassis of the centrifuge.<o:p></o:p></span></p></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4810"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>Similarly, if a botox sample cell leaked, the toxin could go just about anywhere and they would have to throw away the centrifuge: (a second hand apocryphal story from Allergan).<o:p></o:p></span></p></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4810"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4810"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>Kind Regards,<o:p></o:p></span></p></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4810"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4810"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>David<o:p></o:p></span></p></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4810"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p></div><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p><div><p class=MsoNormal style='margin-bottom:12.0pt;background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p></div><div id=yiv6391599244yqt89152><div id="yiv6391599244yui_3_16_0_1_1444844383306_4699"><div id="yiv6391599244yui_3_16_0_1_1444844383306_4698"><div id="yiv6391599244yui_3_16_0_1_1444844383306_4697"><div class=MsoNormal align=center style='text-align:center;background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><hr size=1 width="100%" align=center></span></div><p class=MsoNormal style='background:white'><b><span style='font-size:10.0pt;font-family:"Arial",sans-serif;color:black'>From:</span></b><span style='font-size:10.0pt;font-family:"Arial",sans-serif;color:black'> John Philo <<a href="mailto:jphilo@mailway.com">jphilo@mailway.com</a>><br><b>To:</b> 'David Hayes' <<a href="mailto:drdavidbhayes@yahoo.com">drdavidbhayes@yahoo.com</a>>; 'RASMB' <<a href="mailto:rasmb@rasmb.org">rasmb@rasmb.org</a>> <br><b>Sent:</b> Wednesday, October 14, 2015 1:21 PM<br><b>Subject:</b> RE: [RASMB] Volatile buffers in AUC question</span><span style='font-family:"Helvetica",sans-serif;color:black'><o:p></o:p></span></p></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4704"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p><div id=yiv6391599244><div id="yiv6391599244yui_3_16_0_1_1444844383306_4703"><div id="yiv6391599244yui_3_16_0_1_1444844383306_4702"><div id="yiv6391599244yui_3_16_0_1_1444844383306_4701"><p class=MsoNormal style='background:white'><span style='font-size:10.0pt;font-family:"Helvetica",sans-serif;color:black'>David, if it were leaking into vacuum (no oxygen), how could it burn? Am I missing something?</span><span style='font-family:"Helvetica",sans-serif;color:black'><o:p></o:p></span></p></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4705"><p class=MsoNormal style='background:white'><span style='font-size:10.0pt;font-family:"Helvetica",sans-serif;color:black'> </span><span style='font-family:"Helvetica",sans-serif;color:black'><o:p></o:p></span></p></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4706"><p class=MsoNormal style='background:white'><span style='font-size:10.0pt;font-family:"Helvetica",sans-serif;color:black'>John</span><span style='font-family:"Helvetica",sans-serif;color:black'><o:p></o:p></span></p></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4707"><p class=MsoNormal style='background:white'><span style='font-size:10.0pt;font-family:"Helvetica",sans-serif;color:black'> </span><span style='font-family:"Helvetica",sans-serif;color:black'><o:p></o:p></span></p></div><div id="yui_3_16_0_1_1444844725642_3420"><p class=MsoNormal style='margin-bottom:12.0pt;background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p></div><div id=yiv6391599244yqt97857><div id="yiv6391599244yui_3_16_0_1_1444844383306_4709"><div style='border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in' id="yiv6391599244yui_3_16_0_1_1444844383306_4708"><div id="yiv6391599244yui_3_16_0_1_1444844383306_4710"><p class=MsoNormal style='background:white'><b><span style='font-size:11.0pt;font-family:"Helvetica",sans-serif;color:black'>From:</span></b><span style='font-size:11.0pt;font-family:"Helvetica",sans-serif;color:black'> RASMB [<a href="mailto:rasmb-bounces@list.rasmb.org">mailto:rasmb-bounces@list.rasmb.org</a>] <b>On Behalf Of </b>David Hayes<br><b>Sent:</b> Wednesday, October 14, 2015 9:59 AM<br><b>To:</b> RASMB <<a href="mailto:rasmb@rasmb.org">rasmb@rasmb.org</a>><br><b>Subject:</b> [RASMB] Volatile buffers in AUC question</span><span style='font-family:"Helvetica",sans-serif;color:black'><o:p></o:p></span></p></div></div></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4712"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'> <o:p></o:p></span></p></div><div id="yiv6391599244yui_3_16_0_1_1444844383306_4714"><div id="yiv6391599244yui_3_16_0_1_1444841906429_2631"><div id="yiv6391599244yui_3_16_0_1_1444844383306_4713"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>Hi all,<o:p></o:p></span></p></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2635"><div id="yiv6391599244yui_3_16_0_1_1444844383306_4715"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'> <o:p></o:p></span></p></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2639"><div id="yiv6391599244yui_3_16_0_1_1444844383306_4716"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>I have a sample where we want to run in a buffer with 10% IPA.<o:p></o:p></span></p></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2643"><div id="yiv6391599244yui_3_16_0_1_1444844383306_4718"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>In general, because of the possibility of ignition of flammable components if there was a cell leak, People at AUC workshops say not to run organic solvents in the Beckman XLA/XLI instruments.<o:p></o:p></span></p></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2647"><div id="yui_3_16_0_1_1444844725642_3423"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>Should I be worried about this buffer with 10% isopropyl alcohol? <o:p></o:p></span></p></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2651"><div id="yui_3_16_0_1_1444844725642_3425"><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>Even if a cell leaked and 40 micro liters of IPA evaporated along with the aqueous components, how big of a flash would it be? Could it damage the instrument?<o:p></o:p></span></p></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2655"><div><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'> <o:p></o:p></span></p></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2659"><div><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>I know that polymer chemists also run AUC and wonder if there is any actual experience with organic solvents and any verified incidents of cells leaking and causing fire damage.<o:p></o:p></span></p></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2663"><div><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'> <o:p></o:p></span></p></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2667"><div><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>Kind Regards,<o:p></o:p></span></p></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2671"><div><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'> <o:p></o:p></span></p></div></div><div id="yiv6391599244yui_3_16_0_1_1444841906429_2675"><div><p class=MsoNormal style='background:white'><span style='font-family:"Helvetica",sans-serif;color:black'>David Hayes<o:p></o:p></span></p></div></div></div></div></div></div></div><p class=MsoNormal style='margin-bottom:12.0pt;background:white'><span style='font-family:"Helvetica",sans-serif;color:black'><o:p> </o:p></span></p></div></div></div></div></div></div></div></div></div></body></html>