<html><body><div style="color:#000; background-color:#fff; font-family:HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif;font-size:16px"><div id="yui_3_16_0_1_1433508229354_3434"><span id="yui_3_16_0_1_1433508229354_3550">Please note that I forgot which email was registered to RASMB. This is an exact copy of my previous email, sent earlier, but delayed because of my email mixup.</span></div><div id="yui_3_16_0_1_1433508229354_3434"><span><br></span></div><div id="yui_3_16_0_1_1433508229354_3434" dir="ltr"><span id="yui_3_16_0_1_1433508229354_3565">Thanks to all who have already responded with excellent questions, information and explanations. </span></div><div id="yui_3_16_0_1_1433508229354_3434" dir="ltr"><span><br></span></div><div id="yui_3_16_0_1_1433508229354_3434" dir="ltr"><span id="yui_3_16_0_1_1433508229354_3639">Quite a bit of other work has delayed a full response, though I do have time to say quickly that I forgot Vladmir's paper on using the beads with DLS even though an intern started to implement this here last summer as part of a viscosity project. We have a Wyatt plate reading DLS already, so the reminder is very helpful.</span></div><div id="yui_3_16_0_1_1433508229354_3434" dir="ltr"><span><br></span></div><div id="yui_3_16_0_1_1433508229354_3434" dir="ltr"><span id="yui_3_16_0_1_1433508229354_3677">The response to Steve Harding is also simple: thanks much. It is obvious to me that I need some time to digest and think about intrinsic viscosity more. </span></div><div id="yui_3_16_0_1_1433508229354_3737"><br></div><div id="yui_3_16_0_1_1433508229354_3759" dir="ltr">For John Sumida, I can say that now that I am in research, the final question about viscosity is this: Can and should research throw out some leads because of viscosity problems? </div><div id="yui_3_16_0_1_1433508229354_3759" dir="ltr">Formulation colleagues are being asked to make 100 or 150 mg/ml solutions which must be syringeable. Manufacturing colleagues use lower concentrations (though local concentrations on some filters can get pretty high), but have little flexibility in buffer additions that formulators can use solve some problems. </div><div id="yui_3_16_0_1_1433508229354_3759" dir="ltr">In research the question is whether we can screen viscosity reliably with limited amounts of material and little time to try lots of excipients, and whether or not an otherwise good molecule could have really un-solvable viscosity problems.</div><div id="yui_3_16_0_1_1433508229354_3759" dir="ltr"><br></div><div id="yui_3_16_0_1_1433508229354_3759" dir="ltr">Sorry if I missed anyone by name. I will re-read all the responses eventually.</div><div id="yui_3_16_0_1_1433508229354_3759" dir="ltr"><br></div><div id="yui_3_16_0_1_1433508229354_3759" dir="ltr">Kind Regards,</div><div id="yui_3_16_0_1_1433508229354_3760"><br></div><div id="yui_3_16_0_1_1433508229354_3811">David Hayes</div><div id="yui_3_16_0_1_1433508229354_3810"><br></div> <div style="font-family: HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif; font-size: 16px;" id="yui_3_16_0_1_1433508229354_3414"> <div style="font-family: HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif; font-size: 16px;" id="yui_3_16_0_1_1433508229354_3413"> <div dir="ltr" id="yui_3_16_0_1_1433508229354_3412"> <hr size="1" id="yui_3_16_0_1_1433508229354_3640"> <font size="2" face="Arial" id="yui_3_16_0_1_1433508229354_3433"> <b><span style="font-weight:bold;">From:</span></b> "david.hayes@boehringer-ingelheim.com" <david.hayes@boehringer-ingelheim.com><br> <b><span style="font-weight: bold;">To:</span></b> rasmb@rasmb.org <br> <b><span style="font-weight: bold;">Sent:</span></b> Tuesday, June 2, 2015 12:06 PM<br> <b><span style="font-weight: bold;">Subject:</span></b> [RASMB] Malvern Viscosizer<br> </font> </div> <div class="y_msg_container" id="yui_3_16_0_1_1433508229354_3683"><br><div id="yiv0895182323">
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<div class="yiv0895182323MsoNormal" id="yui_3_16_0_1_1433508229354_3946"><span style="" id="yui_3_16_0_1_1433508229354_3945">Hi all (especially hoping Steve Harding is active),</span></div>
<div class="yiv0895182323MsoNormal" id="yui_3_16_0_1_1433508229354_3950"><span style=""> </span></div>
<div class="yiv0895182323MsoNormal"><span style="">I recently saw the Malvern
<span class="yiv0895182323SpellE">viscosizer</span>.<span style=""> </span>It is a capillary viscometer and
<span class="yiv0895182323SpellE">taylor</span> dispersion instrument.<span style="">
</span>(Taylor dispersion looks at the diffusion pattern of a small plug of sample in a capillary).<span style="">
</span>Because of the small volumes used, it could be useful for screening early limited supply proteins for viscosity problems.</span></div>
<div class="yiv0895182323MsoNormal"><span style=""> </span></div>
<div class="yiv0895182323MsoNormal"><span style="">Are there any general thoughts on the technology?</span></div>
<div class="yiv0895182323MsoNormal"><span style=""> </span></div>
<div class="yiv0895182323MsoNormal"><span style="">I remember Steve Harding telling us at BITC that you never use rolling ball viscometers on proteins:<span style="">
</span>the proteins stick to the ball.</span></div>
<div class="yiv0895182323MsoNormal"><span style="">Recently we found out here that the regular Anton
<span class="yiv0895182323SpellE">Paar</span> cone and plate <span class="yiv0895182323SpellE">rheometers</span> sometimes need PS-80 in protein samples or the air water interface around the outside edge can predominate the viscosity measurements.</span></div>
<div class="yiv0895182323MsoNormal"><span style=""> </span></div>
<div class="yiv0895182323MsoNormal" id="yui_3_16_0_1_1433508229354_3686"><span style="" id="yui_3_16_0_1_1433508229354_3685">I did ask whether most samples just stick to the capillary and disappear or run anomalously, but the answer was they had a super coating so most things won’t stick.<span style="">
</span>This coating makes the capillary impossible to clean (so you buy more capillaries from Malvern).<span style="">
</span>And that the <span class="yiv0895182323SpellE">taylor</span> dispersion fitting will tell you if and approximately how much the sample interacts with the capillary walls.</span></div>
<div class="yiv0895182323MsoNormal"><span style=""> </span></div>
<div class="yiv0895182323MsoNormal"><span style="">Kind Regards,</span></div>
<div class="yiv0895182323MsoNormal"><span style=""> </span></div>
<div class="yiv0895182323MsoNormal"><span style="">David Hayes</span></div>
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