<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40"><head><meta http-equiv=Content-Type content="text/html; charset=us-ascii"><meta name=Generator content="Microsoft Word 15 (filtered medium)"><style><!--
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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link=blue vlink=purple><div class=WordSection1><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial",sans-serif;color:blue'>Peter, I may be mistaken, but I think the modern Beckman preparative centrifuges (the ones with a PC internally) may have software that does those calculations.<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial",sans-serif;color:blue'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial",sans-serif;color:blue'>John<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial",sans-serif;color:blue'><o:p> </o:p></span></p><div><div style='border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in'><p class=MsoNormal><b>From:</b> RASMB [mailto:rasmb-bounces@list.rasmb.org] <b>On Behalf Of </b>Peter Edward Prevelige Jr<br><b>Sent:</b> Friday, April 24, 2015 12:06 PM<br><b>To:</b> rasmb@rasmb.org<br><b>Subject:</b> [RASMB] Isopycnic Help<o:p></o:p></p></div></div><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Hi RASMB-ers<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Centrifuge but not AU related – <o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>I need to set up CsCl gradients with the greatest possible density resolution in a narrow range (i.e. shallow gradient about a defined middle value). I’d like a facile way to model the gradients formed at different rotor speeds and initial CsCl densities. Does anyone know of a program that will allow me to do this easily. <o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Thanks<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><span style='font-size:10.0pt;color:#1F497D'>Peter E. Prevelige Jr.</span><span style='font-size:10.0pt'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;color:#1F497D'>Professor</span><span style='font-size:10.0pt'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;color:#1F497D'>Dept. of Microbiology, BBRB 416/6</span><span style='font-size:10.0pt'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;color:#1F497D'>Univ. of Alabama @ Birmingham</span><span style='font-size:10.0pt'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;color:#1F497D'>845 19th St. South</span><span style='font-size:10.0pt'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;color:#1F497D'>Birmingham AL. 35294-2170</span><span style='font-size:10.0pt'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;color:#1F497D'>Phone 205 975-5327</span><span style='font-size:10.0pt'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;color:#1F497D'>FAX 205 975-5479</span><span style='font-size:10.0pt'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;color:#1F497D'><a href="mailto:prevelig@uab.edu">prevelig@uab.edu</a></span><span style='font-size:10.0pt'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;color:#1F497D'><a href="http://www.preveligelab.org">http://www.preveligelab.org</a></span><span style='font-size:10.0pt'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.5pt;font-family:Consolas;color:#1F497D'> </span><o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p></div></body></html>