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<DIV dir=ltr align=left><FONT color=#0000ff size=2 face=Arial><SPAN
class=525385418-18122014>The other issue with the meniscus matching centerpeices
is to be careful to not overfill the buffer channel higher than the top
capillary. This prevents equalization of the pressures between the two
channels, and can inhibit the desired meniscus
matching.</SPAN></FONT></DIV>
<DIV><FONT color=#0000ff size=2 face=Arial><SPAN
class=525385418-18122014></SPAN></FONT> </DIV>
<DIV><FONT color=#0000ff size=2 face=Arial><SPAN
class=525385418-18122014>Karl</SPAN></FONT></DIV>
<DIV dir=ltr align=left><BR></DIV>
<DIV lang=en-us class=OutlookMessageHeader dir=ltr align=left>
<HR tabIndex=-1>
<FONT size=2 face=Tahoma><B>From:</B> RASMB
[mailto:rasmb-bounces@list.rasmb.org] <B>On Behalf Of </B>John
Philo<BR><B>Sent:</B> Thursday, December 18, 2014 10:49 AM<BR><B>To:</B> 'John
Sumida'; rasmb@rasmb.org<BR><B>Subject:</B> Re: [RASMB] Centerpiece
question<BR></FONT><BR></DIV>
<DIV></DIV>
<DIV class=WordSection1>
<P class=MsoNormal><SPAN
style='FONT-SIZE: 10pt; FONT-FAMILY: "Arial",sans-serif; COLOR: blue'>John, in
my view the advantages of the Stafford meniscus-matching design (available from
Spin Analytical) over the synthetic boundary centerpieces are that the long
capillary design inhibits diffusion between the two channels and any inadvertent
fluid transfer during loading and handling. The drawback of that long channel
though is that it is harder to get the transfer needed to match the heights (it
takes more force)---I find I may need to go to 10-12 K rpm. For some of the
virus samples we run that is >= to the run speed, so you have to do it very
quickly to avoid significant sedimentation during the meniscus matching
spin.<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN
style='FONT-SIZE: 10pt; FONT-FAMILY: "Arial",sans-serif; COLOR: blue'><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN
style='FONT-SIZE: 10pt; FONT-FAMILY: "Arial",sans-serif; COLOR: blue'>John<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN
style='FONT-SIZE: 10pt; FONT-FAMILY: "Arial",sans-serif; COLOR: blue'><o:p> </o:p></SPAN></P>
<DIV>
<DIV
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<P class=MsoNormal><B>From:</B> RASMB [mailto:rasmb-bounces@list.rasmb.org]
<B>On Behalf Of </B>John Sumida<BR><B>Sent:</B> Thursday, December 18, 2014 9:45
AM<BR><B>To:</B> rasmb@rasmb.org<BR><B>Subject:</B> [RASMB] Centerpiece
question<o:p></o:p></P></DIV></DIV>
<P class=MsoNormal><o:p> </o:p></P>
<P class=MsoNormal><SPAN
style='FONT-SIZE: 12pt; FONT-FAMILY: "Courier New"'>Dear
RASMB,<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN
style='FONT-SIZE: 12pt; FONT-FAMILY: "Courier New"'><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN style='FONT-SIZE: 12pt; FONT-FAMILY: "Courier New"'>For
interference measurements where matching the sample and reference channel
menisci is essential, is there a preference for either the meniscus matching
centerpiece versus synthetic boundary centerpiece. We would be interested
to hear about pros and cons.<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN
style='FONT-SIZE: 12pt; FONT-FAMILY: "Courier New"'><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN
style='FONT-SIZE: 12pt; FONT-FAMILY: "Courier New"'>Thank you and happy
holidays.<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN
style='FONT-SIZE: 12pt; FONT-FAMILY: "Courier New"'><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN
style='FONT-SIZE: 12pt; FONT-FAMILY: "Courier New"'>Best
regards,<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN
style='FONT-SIZE: 12pt; FONT-FAMILY: "Courier New"'><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN
style='FONT-SIZE: 12pt; FONT-FAMILY: "Courier New"'>John Sumida,
Ph.D.<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style='FONT-SIZE: 12pt; FONT-FAMILY: "Courier New"'><A
href="http://depts.washington.edu/cidb4bio/index.shtml"><SPAN
style="COLOR: windowtext">Analytical Biopharmacy Core
Facility</SPAN></A><o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN
style='FONT-SIZE: 12pt; FONT-FAMILY: "Courier New"'>University of
Washington<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style='FONT-SIZE: 12pt; FONT-FAMILY: "Courier New"'><A
href="http://www.moles.washington.edu/"><SPAN
style="COLOR: windowtext">Molecular Engineering & Sciences Institute,
G22</SPAN></A><o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN
style='FONT-SIZE: 12pt; FONT-FAMILY: "Courier New"'>3946 West Stevens Way
NE<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN
style='FONT-SIZE: 12pt; FONT-FAMILY: "Courier New"'>Seattle WA
98195-1653<o:p></o:p></SPAN></P>
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