<html><head><meta http-equiv="Content-Type" content="text/html charset=windows-1252"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Hi John,<div><span class="Apple-tab-span" style="white-space:pre"> </span>The synthetic boundary centerpieces generally work OK. A potential problem is that some protein may transfer from the solution side to the reference either by diffusion or by sedimentation (i.e. if the capillary is slightly "downhill" from the samples side to the reference side.)</div><div><br></div><div>The MM centerpieces wer designed to avoid that potential problem. If you want to make your own MM centerpieces, you can scribe a capillary on the center rib with a deliberate "uphill" slant to avoid missing - this must be done very carefully under a dissecting microscope with a sharp, pointy object and must not be "too" deep, etc, etc... And no caffeine ...</div><div><br></div><div>Good luck,</div><div>Walter</div><div><br></div><div><br><div><div>On Dec 18, 2014, at 12:44, John Sumida <<a href="mailto:jpsumida@u.washington.edu">jpsumida@u.washington.edu</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><meta http-equiv="Content-Type" content="text/html; charset=us-ascii"><meta name="Generator" content="Microsoft Word 14 (filtered medium)"><style><!--
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</o:shapelayout></xml><![endif]--><div lang="EN-US" link="blue" vlink="purple"><div class="WordSection1"><p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Courier New"">Dear RASMB,<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Courier New""> </span></p><p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Courier New"">For interference measurements where matching the sample and reference channel menisci is essential, is there a preference for either the meniscus matching centerpiece versus synthetic boundary centerpiece. We would be interested to hear about pros and cons.<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Courier New""> </span></p><p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Courier New"">Thank you and happy holidays.<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Courier New""> </span></p><p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Courier New"">Best regards,<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Courier New""> </span></p><p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Courier New"">John Sumida, Ph.D.<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Courier New""><a href="http://depts.washington.edu/cidb4bio/index.shtml"><span style="color:windowtext">Analytical Biopharmacy Core Facility</span></a><o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Courier New"">University of Washington<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Courier New""><a href="http://www.moles.washington.edu/"><span style="color:windowtext">Molecular Engineering & Sciences Institute, G22</span></a><o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Courier New"">3946 West Stevens Way NE<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Courier New"">Seattle WA 98195-1653<o:p></o:p></span></p><p class="MsoNormal" style="text-autospace:none"><span style=""> </span></p></div></div>_______________________________________________<br>RASMB mailing list<br><a href="mailto:RASMB@list.rasmb.org">RASMB@list.rasmb.org</a><br>http://list.rasmb.org/listinfo.cgi/rasmb-rasmb.org<br></blockquote></div><br><div apple-content-edited="true">
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Arial; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><br class="Apple-interchange-newline">Walter Stafford<br><a href="mailto:wstafford3@walterstafford.com">wstafford3@walterstafford.com</a><br><br>"We're all better off when we're all better off" –Eric Liu</span>
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